Abstract |
We assessed the effect of β- Glucan on macrophages by Griess reagent and viability by MTT assay and cytotoxicity. Assay of macrophages culture supernatants were carried out on WEHI-164 fibrosarcoma cell line as tumor necrosis factor-α bioassay were done. NO release was increased at the dose of 10 µg/ml (P = 0.001) of β- Glucan while the viability of macrophages in all concentrations was the same. In TNF-α bioassay, the supernatant of macrophages stimulated with β- Glucan had a significant cytotoxic effect on WEHI-164 cells (P = 0.023). β- Glucan had a positive effect on increasing tumoricidal activity of macrophages which may help in anti- cancer immune responses.
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Authors | Maryam Roudbary, Saeed Daneshmand, Monire Hajimorad, Shahla Roudbarmohammadip, Zuhair M Hassan |
Journal | Polish journal of microbiology
(Pol J Microbiol)
Vol. 64
Issue 2
Pg. 175-9
( 2015)
ISSN: 1733-1331 [Print] Poland |
PMID | 26373179
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Cells, Cultured
- Dose-Response Relationship, Drug
- Female
- Macrophages, Peritoneal
(drug effects)
- Mice
- Mice, Inbred BALB C
- beta-Glucans
(pharmacology)
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