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Modification of prostaglandin synthesis in washed human platelets and cultured bovine aortic endothelial cells by glycosylated low density lipoprotein.

Abstract
Glycosylation of low-density lipoprotein (LDL) is known to be increased in diabetic patients. Recent studies have demonstrated that glycosylated (glc-) LDL contributes to the acceleration of atherosclerosis in diabetes. In the present study, we evaluated the effect of glc-LDL prepared in vitro on platelet aggregation and thromboxane B2 (TxB2) production in washed human platelets and on 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) production by cultured bovine aortic endothelial cells. After preincubation of washed platelets with glc-LDL or control LDL, thrombin-induced platelet aggregation and TxB2 production were measured. Control LDL enhanced the platelet aggregation rate and TxB2 production in a time- and dose-dependent manner. Glc-LDL showed significantly greater enhancement of those platelet functions than control LDL. On the other hand, while 6-keto-PGF1 alpha production was stimulated by control LDL in a time- and dose-dependent manner, glc-LDL significantly reduced the stimulatory effect of control LDL on 6-keto-PGF1 alpha production. These results suggest that modification of prostaglandin synthesis in platelets and endothelial cells by glycosylated LDL may lead to platelet hyperaggregation and thrombus formation in diabetes.
AuthorsH Ishii, F Umeda, M Kunisaki, T Yamauchi, H Nawata
JournalDiabetes research (Edinburgh, Scotland) (Diabetes Res) Vol. 12 Issue 4 Pg. 177-82 (Dec 1989) ISSN: 0265-5985 [Print] Scotland
PMID2637093 (Publication Type: Journal Article)
Chemical References
  • Glycation End Products, Advanced
  • Lipoproteins, LDL
  • Prostaglandins
  • glycated lipoproteins, LDL
  • Thromboxane B2
  • 6-Ketoprostaglandin F1 alpha
Topics
  • 6-Ketoprostaglandin F1 alpha (biosynthesis)
  • Animals
  • Blood Platelets (drug effects, metabolism)
  • Cattle
  • Cells, Cultured
  • Endothelium, Vascular (drug effects, metabolism)
  • Glycation End Products, Advanced
  • Glycosylation
  • Humans
  • In Vitro Techniques
  • Kinetics
  • Lipoproteins, LDL (blood, pharmacology)
  • Platelet Aggregation
  • Prostaglandins (biosynthesis, blood)
  • Thromboxane B2 (biosynthesis, blood)

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