Abstract |
Glycosylation of low-density lipoprotein ( LDL) is known to be increased in diabetic patients. Recent studies have demonstrated that glycosylated ( glc-) LDL contributes to the acceleration of atherosclerosis in diabetes. In the present study, we evaluated the effect of glc-LDL prepared in vitro on platelet aggregation and thromboxane B2 (TxB2) production in washed human platelets and on 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) production by cultured bovine aortic endothelial cells. After preincubation of washed platelets with glc-LDL or control LDL, thrombin-induced platelet aggregation and TxB2 production were measured. Control LDL enhanced the platelet aggregation rate and TxB2 production in a time- and dose-dependent manner. Glc-LDL showed significantly greater enhancement of those platelet functions than control LDL. On the other hand, while 6-keto-PGF1 alpha production was stimulated by control LDL in a time- and dose-dependent manner, glc-LDL significantly reduced the stimulatory effect of control LDL on 6-keto-PGF1 alpha production. These results suggest that modification of prostaglandin synthesis in platelets and endothelial cells by glycosylated LDL may lead to platelet hyperaggregation and thrombus formation in diabetes.
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Authors | H Ishii, F Umeda, M Kunisaki, T Yamauchi, H Nawata |
Journal | Diabetes research (Edinburgh, Scotland)
(Diabetes Res)
Vol. 12
Issue 4
Pg. 177-82
(Dec 1989)
ISSN: 0265-5985 [Print] Scotland |
PMID | 2637093
(Publication Type: Journal Article)
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Chemical References |
- Glycation End Products, Advanced
- Lipoproteins, LDL
- Prostaglandins
- glycated lipoproteins, LDL
- Thromboxane B2
- 6-Ketoprostaglandin F1 alpha
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Topics |
- 6-Ketoprostaglandin F1 alpha
(biosynthesis)
- Animals
- Blood Platelets
(drug effects, metabolism)
- Cattle
- Cells, Cultured
- Endothelium, Vascular
(drug effects, metabolism)
- Glycation End Products, Advanced
- Glycosylation
- Humans
- In Vitro Techniques
- Kinetics
- Lipoproteins, LDL
(blood, pharmacology)
- Platelet Aggregation
- Prostaglandins
(biosynthesis, blood)
- Thromboxane B2
(biosynthesis, blood)
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