A knot polymer mediated non-viral gene transfection for skin cells.

Abstract

A knot polymer, poly[bis(2-acryloyl)oxyethyl disulphide-co-2-(dimethylamino) ethyl methacrylate] (DSP), was synthesized, optimized and evaluated as a non-viral vector for gene transfection for skin cells, keratinocytes. With recessive dystrophic epidermolysis bullosa keratinocytes (RDEBK-TA4), the DSP exhibited high transfection efficacy with both Gaussia luciferase marker DNA and the full length COL7A1 transcript encoding the therapeutic type VII collagen protein (C7). The effective restoration of C7 in C7 null-RDEB skin cells indicates that DSP is promising for non-viral gene therapy of recessive dystrophic epidermolysis bullosa (RDEB).

• Authors: Lara Cutlar, Yongsheng Gao, Ahmed Aied, Udo Greiser, Eva Maria Murauer, Dezhong Zhou, Wenxin Wang
• Journal: Biomaterials science (Biomater Sci) Vol. 4 Issue 1 Pg. 92-5 (Jan 2016) ISSN: 2047-4849 [Electronic] England
• PMID: 26369723 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Collagen Type VII
• DNA, Complementary
• Dimethylamines
• Methacrylates
• Polymers
• poly(bis(2-acryloyl)oxyethyl disulphide-co-2-(dimethylamino)ethyl methacrylate

Topics

• Collagen Type VII (chemistry, genetics, metabolism)
• DNA, Complementary (genetics)
• Dimethylamines (chemical synthesis, chemistry)
• Epidermolysis Bullosa Dystrophica (genetics, metabolism)
• Gene Transfer Techniques
• Genetic Therapy (methods)
• Genetic Vectors (chemistry, metabolism)
• Humans
• Methacrylates (chemical synthesis, chemistry)
• Polymers (chemistry, metabolism)
• Skin (chemistry, metabolism)
• Transfection