Punicalagin, an active component in pomegranate, ameliorates cardiac mitochondrial impairment in obese rats via AMPK activation.

Abstract

Obesity is associated with an increasing prevalence of cardiovascular diseases and metabolic syndrome. It is of paramount importance to reduce obesity-associated cardiac dysfunction and impaired energy metabolism. In this study, the activation of the AMP-activated protein kinase (AMPK) pathway by punicalagin (PU), a major ellagitannin in pomegranate was investigated in the heart of a rat obesity model. In male SD rats, eight-week administration of 150 mg/kg pomegranate extract (PE) containing 40% punicalagin sufficiently prevented high-fat diet (HFD)-induced obesity associated accumulation of cardiac triglyceride and cholesterol as well as myocardial damage. Concomitantly, the AMPK pathway was activated, which may account for prevention of mitochondrial loss via upregulating mitochondrial biogenesis and amelioration of oxidative stress via enhancing phase II enzymes in the hearts of HFD rats. Together with the normalized expression of uncoupling proteins and mitochondrial dynamic regulators, PE significantly prevented HFD-induced cardiac ATP loss. Through in vitro cultures, we showed that punicalagin was the predominant component that activated AMPK by quickly decreasing the cellular ATP/ADP ratio specifically in cardiomyocytes. Our findings demonstrated that punicalagin, the major active component in PE, could modulate mitochondria and phase II enzymes through AMPK pathway to prevent HFD-induced cardiac metabolic disorders.

• Authors: Ke Cao, Jie Xu, Wenjun Pu, Zhizhong Dong, Lei Sun, Weijin Zang, Feng Gao, Yong Zhang, Zhihui Feng, Jiankang Liu
• Journal: Scientific reports (Sci Rep) Vol. 5 Pg. 14014 (Sep 15 2015) ISSN: 2045-2322 [Electronic] England
• PMID: 26369619 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydrolyzable Tannins
• punicalagin
• AMP-Activated Protein Kinases

Topics

• AMP-Activated Protein Kinases (metabolism)
• Animals
• Diet, High-Fat
• Hydrolyzable Tannins (pharmacology)
• Lythraceae (chemistry)
• Mitochondria, Heart (drug effects, metabolism)
• Myocardium (metabolism)
• Myocytes, Cardiac (drug effects, metabolism)
• Obesity (metabolism)
• Organelle Biogenesis
• Oxidative Stress
• Phosphorylation
• Rats
• Signal Transduction (drug effects)