Abstract |
Peptidoglycan recognition proteins (PGRPs) are members of the innate immune system that interact with bacteria by binding to bacterial peptidoglycan. In this study, we examined the expression and function of a short type of PGRP, CsPGRP-SC2, from tongue sole (Cynoglossus semilaevis). CsPGRP-SC2 contains 164 amino acid residues and shares 54.5%-65.3% overall sequence identities with other teleost PGRPs. CsPGRP-SC2 possesses an amidase domain with a conserved zinc binding site. CsPGRP-SC2 expression occurred in multiple tissues and was upregulated by bacterial and viral infection. Purified recombinant CsPGRP-SC2 (rCsPGRP-SC2) was able to bind and agglutinate Gram-positive bacteria in a Zn(2+)-dependent manner. rCsPGRP-SC2 enhanced the uptake of the bound bacteria by host phagocytes and reduced bacterial dissemination and colonization in host tissues. These results indicate that CsPGRP-SC2 is an innate immune factor that participates in host defense against bacterial infection.
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Authors | Qing-lei Sun, Li Sun |
Journal | Fish & shellfish immunology
(Fish Shellfish Immunol)
Vol. 47
Issue 1
Pg. 313-20
(Nov 2015)
ISSN: 1095-9947 [Electronic] England |
PMID | 26364742
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ltd. All rights reserved. |
Chemical References |
- Carrier Proteins
- Fish Proteins
- peptidoglycan recognition protein
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Topics |
- Amino Acid Sequence
- Animals
- Carrier Proteins
(chemistry, genetics, metabolism)
- DNA Virus Infections
(genetics, immunology, veterinary, virology)
- Fish Diseases
(genetics, immunology, microbiology, virology)
- Fish Proteins
(chemistry, genetics, metabolism)
- Flatfishes
- Immunity, Innate
- Iridoviridae
(physiology)
- Phagocytosis
- Real-Time Polymerase Chain Reaction
(veterinary)
- Sequence Alignment
(veterinary)
- Streptococcal Infections
(genetics, immunology, microbiology, veterinary)
- Streptococcus
(physiology)
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