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(1)H NMR metabolomics to study the effects of diazepam on anisatin induced convulsive seizures.

Abstract
The anticonvulsive properties of diazepam have been extensively studied, mainly focusing on the γ-amino butyrate (GABA) system. The aim of this investigation was to integrally analyze the metabolic events related to neuroprotection of diazepam on anisatin-induced convulsive seizures by a NMR-based metabolomic approach combined with histopathological examination and behavior examination. Multivariate analysis on metabolic profiles of the piriform cortex and cerebellum of mice revealed that diazepam could relieve mice suffering from the convulsive seizures by recovering destructed neurotransmitter and neuromodulator metabolism, ameliorating oxidative stress, alleviating the disturbance in energy, amino acid and nucleic acid metabolism in anisatin intoxicated mice. This integrated metabolomics study provided a powerful and highly effective approach to elucidate therapeutic effects and assessed the safety of diazepam. This study should be helpful for our understanding of convulsive seizures, and provide a holistic view of the treatment effects of benzodiazepine on convulsive seizures.
AuthorsPei Li, Dan-Dan Wei, Jun-Song Wang, Ming-Hua Yang, Ling-Yi Kong
JournalJournal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 117 Pg. 184-94 (Jan 05 2016) ISSN: 1873-264X [Electronic] England
PMID26361344 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Elsevier B.V. All rights reserved.
Chemical References
  • Lactones
  • Protons
  • Sesquiterpenes
  • Spiro Compounds
  • Diazepam
  • anisatin
Topics
  • Animals
  • Brain (drug effects, metabolism)
  • Diazepam (pharmacology, therapeutic use)
  • Illicium
  • Lactones (toxicity)
  • Male
  • Metabolomics (methods)
  • Mice
  • Mice, Inbred ICR
  • Nuclear Magnetic Resonance, Biomolecular (methods)
  • Protons
  • Seizures (chemically induced, drug therapy, metabolism)
  • Sesquiterpenes (toxicity)
  • Spiro Compounds (toxicity)

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