MicroRNA-203-mediated posttranscriptional deregulation of CPEB4 contributes to colorectal cancer progression.

Abstract

Elevated cytoplasmic polyadenylation element-binding 4 (CPEB4) is aberrantly expressed in several malignant cancers. However, its expression pattern, clinical significance, and biological function in colorectal cancer are still unknown. In this study, we demonstrated that CPEB4 is abundantly overexpressed in colorectal cancers and has the potential to be used for predicting clinical outcomes of colorectal cancer patients. We suppressed CPEB4 expression by small interfering RNA (siRNA) in SW480 and LOVO cells to clarify the role of CPEB4 on the cell apoptosis and proliferation in vitro. Further study revealed that knockdown of CPEB4 decreased the expression of anti-apoptotic protein B-cell lymphoma-extra large (Bcl-XL), but enhanced the expression of B-cell lymphoma-2-associated X (Bax). In addition, we indicated that CPEB4 is a novel target of miR-203, a tumor suppressive microRNA. Notably, restoration of CPEB4 in SW480 cells inhibited miR-203-induced apoptosis signaling pathway, which in turn enhanced cell proliferation and suppressed cell apoptosis. Taken together, our findings imply that posttranscriptional deregulation of CPEB4 contributes to the inhibited cell proliferation and the enhanced cell apoptosis in colorectal cancer, and directly targeting CPEB4 by miR-203 might be a novel strategy in colorectal cancer treatment.

• Authors: Xiaohua Zhong, Yipin Xiao, Chao Chen, Xiuwen Wei, Chen Hu, Xukun Ling, Xinbin Liu
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 466 Issue 2 Pg. 206-13 (Oct 16 2015) ISSN: 1090-2104 [Electronic] United States
• PMID: 26361147 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• CPEB4 protein, human
• MIRN203 microRNA, human
• MicroRNAs
• RNA-Binding Proteins

Topics

• Colorectal Neoplasms (genetics, pathology)
• Disease Progression
• Female
• Humans
• Male
• MicroRNAs (physiology)
• Middle Aged
• RNA Processing, Post-Transcriptional
• RNA-Binding Proteins (genetics)