Chemotactic effect of PAF-acether on peritoneal eosinophils from normal rats.

Abstract

The chemotactic activity of PAF-acether was compared with that of tetrapeptide eosinophil chemotactic factors of anaphylaxis (ECF-A, Ala-Gly-Ser-Glu and Val-Gly-Ser-Glu) using eosinophils obtained from the peritoneal cavity of normal rats. Cells were isolated by separation over discontinuous metrizamide gradients which resulted in eosinophil suspensions of 80 to 90% purity. PAF-acether produced a dose-dependent effect which, at its maximum, was more than 30-fold greater than control and about 5- to 7-fold greater than the maximal activity obtained with the ECF-A-tetrapeptides. BN 52021 and WEB 2086 inhibited PAF-acether-induced eosinophil chemotaxis in a dose-dependent manner, suggesting that this phenomenon is mediated by specific PAF-acether receptors.

• Authors: M A Martins, A Etienne, C Soulard, M T Domingo, P Braquet
• Journal: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (Braz J Med Biol Res) Vol. 22 Issue 9 Pg. 1151-4 ( 1989) ISSN: 0100-879X [Print] Brazil
• PMID: 2636011 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Azepines
• Chemotactic Factors
• Chemotactic Factors, Eosinophil
• Diterpenes
• Ginkgolides
• Lactones
• Platelet Activating Factor
• Triazoles
• WEB 2086
• ginkgolide B

Topics

• Animals
• Azepines (pharmacology)
• Chemotactic Factors (pharmacology)
• Chemotactic Factors, Eosinophil (pharmacology)
• Chemotaxis, Leukocyte (drug effects)
• Diterpenes
• Eosinophils (physiology)
• Ginkgolides
• In Vitro Techniques
• Lactones (pharmacology)
• Male
• Peritoneal Cavity (cytology)
• Platelet Activating Factor (antagonists & inhibitors, pharmacology)
• Rats
• Rats, Inbred Strains
• Triazoles (pharmacology)