Abstract |
Small heat shock proteins (sHSPs) perform a fundamental role in protecting cells against a wide array of stresses but their biological function during viral infection remains unknown. Rice stripe virus (RSV) causes a severe disease of rice in Eastern Asia. OsHSP20 and its homologue (NbHSP20) were used as baits in yeast two-hybrid (YTH) assays to screen an RSV cDNA library and were found to interact with the viral RNA-dependent RNA polymerase (RdRp) of RSV. Interactions were confirmed by pull-down and BiFC assays. Further analysis showed that the N-terminus (residues 1-296) of the RdRp was crucial for the interaction between the HSP20s and viral RdRp and responsible for the alteration of the sub-cellular localization and distribution pattern of HSP20s in protoplasts of rice and epidermal cells of Nicotiana benthamiana. This is the first report that a plant virus or a viral protein alters the expression pattern or sub-cellular distribution of sHSPs.
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Authors | Jing Li, Cong-Ying Xiang, Jian Yang, Jian-Ping Chen, Heng-Mu Zhang |
Journal | Scientific reports
(Sci Rep)
Vol. 5
Pg. 14016
(Sep 11 2015)
ISSN: 2045-2322 [Electronic] England |
PMID | 26359114
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HSP20 Heat-Shock Proteins
- Plant Proteins
- Viral Proteins
- RNA-Dependent RNA Polymerase
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Topics |
- Gene Expression Regulation, Plant
- HSP20 Heat-Shock Proteins
(genetics, metabolism)
- Host-Pathogen Interactions
- Intracellular Space
(metabolism)
- Oryza
(metabolism, virology)
- Plant Diseases
(genetics, virology)
- Plant Proteins
(metabolism)
- Plants
(genetics, metabolism, virology)
- Protein Binding
- Protein Interaction Domains and Motifs
(genetics)
- Protein Transport
- RNA-Dependent RNA Polymerase
(chemistry, metabolism)
- Viral Proteins
(metabolism)
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