Role of Inhibitors of Apoptosis Proteins in Testicular Function and Male Fertility: Effects of Polydeoxyribonucleotide Administration in Experimental Varicocele.

Abstract

Neuronal apoptosis inhibitory protein (NAIP) and survivin might play an important role in testicular function. We investigated the effect of PDRN, an agonist of adenosine A2A receptor, on testicular NAIP and survivin expression in an experimental model of varicocele. After the creation of experimental varicocele (28 days), adolescent male Sprague-Dawley rats were randomized to one of the following treatments lasting 21 days: vehicle, PDRN (8 mg/kg i.p., daily), PDRN + 3,7-dimethyl-propargylxanthine (DMPX, a specific adenosine A2A-receptor antagonist, 0.1 mg/kg i.p., daily), varicocelectomy, and varicocelectomy + PDRN (8 mg/kg i.p., daily). Sham-operated animals were used as controls. Animals were then euthanized and testis expression of NAIP and survivin was evaluated through qRT-PCR, western blot, and immunohistochemical analysis. Spermatogenetic activity was also assessed. NAIP and survivin expressions were significantly reduced following varicocele induction when compared to sham animals whereas PDRN-treated rats showed an increase in NAIP and survivin levels. Immunohistochemistry revealed an enhanced expression of NAIP and survivin with a characteristic pattern of cellular localization following PDRN treatment. Moreover, administration of PDRN significantly restored spermatogenic function in varicocele rats. PDRN may represent a rational therapeutic option for accelerating recovery from depressed testicular function through a strategic modulation of apoptosis in experimental varicocele.

• Authors: Letteria Minutoli, Salvatore Arena, Pietro Antonuccio, Carmelo Romeo, Alessandra Bitto, Carlo Magno, Mariagrazia Rinaldi, Antonio Micali, Natasha Irrera, Gabriele Pizzino, Federica Galfo, Francesco Squadrito, Domenica Altavilla, Herbert Marini
• Journal: BioMed research international (Biomed Res Int) Vol. 2015 Pg. 248976 ( 2015) ISSN: 2314-6141 [Electronic] United States
• PMID: 26347229 (Publication Type: Journal Article)

Chemical References

• Adenosine A2 Receptor Antagonists
• Birc5 protein, rat
• Microtubule-Associated Proteins
• Naip6 protein, rat
• Neuronal Apoptosis-Inhibitory Protein
• Oligodeoxyribonucleotides
• Receptors, Adenosine A2
• Survivin
• 3,7-dimethyl-1-propargylxanthine
• Theobromine

Topics

• Adenosine A2 Receptor Antagonists (pharmacology)
• Animals
• Disease Models, Animal
• Fertility (drug effects)
• Gene Expression Regulation (drug effects)
• Male
• Microtubule-Associated Proteins (biosynthesis)
• Neuronal Apoptosis-Inhibitory Protein (biosynthesis)
• Oligodeoxyribonucleotides (pharmacology)
• Rats
• Receptors, Adenosine A2 (metabolism)
• Spermatogenesis (drug effects)
• Survivin
• Testis (metabolism, pathology)
• Theobromine (analogs & derivatives, pharmacology)
• Varicocele (metabolism, pathology)