CpG ODN 1826 enhances radiosensitivity of the human lung cancer cell line A549 in a rat model.

Abstract

This study investigated the effects of CpG ODN1826 plus radiotherapy (RT) on tumor growth and angiogenesis of subcutaneous tumor in a rat model. Four treatment groups were tested in which rats were injected with 100 μL CpG ODN1826 (1 μg/μL) or 100 μL vehicle, with and without exposure to 8 Gy after 2 h. At 7 days after inoculation of lung cancer cells, drugs were injected in the tumor and radiation was administered over 5 days, after which the rate of tumor inhibition was calculated. Expression of VEGF-C in tumor tissue was seen in 10, 50, 80, and 100% of tumors in the CpG ODN1826 + RT, CpG ODN1826, vehicle + RT, and vehicle alone groups, respectively, while positive expression of NRP-1 was seen in 10, 40, 90, and 100% of tumors. The decreases in expression of VEGF-C mRNA in the CpG ODN1826 + RT and CpG ODN1826 groups compared with the NS + RT and NS groups were significant (P < 0.01), as were the decreases in NRP-1 mRNA in the CpG ODN1826 + RT group compared with the CpG ODN1826 group (P < 0.01). Thus, CpG ODN1826 can significantly inhibit tumor growth in a rat model, the mechanism of which may be related to inhibition of the expression of VEGF-C and NRP-1, which have an inhibitory effect on angiogenesis.

• Authors: W Yan, T-Y Sun, C-M Yang, M Jia, J Li, H-L Tang, Y-F Zhou
• Journal: Genetics and molecular research : GMR (Genet Mol Res) Vol. 14 Issue 3 Pg. 9804-12 (Aug 19 2015) ISSN: 1676-5680 [Electronic] Brazil
• PMID: 26345913 (Publication Type: Journal Article)

Chemical References

• CpG ODN 1826
• Oligodeoxyribonucleotides
• RNA, Messenger
• Vascular Endothelial Growth Factor C
• Neuropilin-1

Topics

• Animals
• Cell Line, Tumor
• Disease Models, Animal
• Gene Expression
• Humans
• Lung Neoplasms (genetics, pathology, therapy)
• Neuropilin-1 (genetics)
• Oligodeoxyribonucleotides (administration & dosage, pharmacology)
• RNA, Messenger (genetics)
• Radiation Tolerance (drug effects)
• Rats
• Tumor Burden (drug effects, radiation effects)
• Vascular Endothelial Growth Factor C (genetics)
• Xenograft Model Antitumor Assays