Abstract | CONTEXT: OBJECTIVE: MATERIALS AND METHODS: Experimental animals divided into four groups (n = 10): the healthy group (HG), the ethambutol control group (EMB), thiamine + ethambutol group (Thi-EMB) and thiamine pyrophosphate + ethambutol group (TPP-EMB). The rats in the TPP-EMB and Thi-EMB groups were administered thiamine pyrophosphate and thiamine, respectively, at doses of 20 mg/kg intraperitoneally. Distilled water was administered intraperitoneally to the HG and the EMB groups as a solvent in the same volumes. One hour after drug injection, 30 mg/kg ethambutol was administered via an oral gavage to the TPP-EMB, Thi-EMB and EMB groups. This procedure was repeated once a day for 90 days. At the end of this period, all rats were euthanized under high-dose thiopental sodium anesthesia, and biochemical and histopathological investigations of the retinal tissue were performed. RESULTS:
Malondialdehyde (MDA) and DNA damage product 8-hydroxyguanine levels were significantly lower in the retinal tissue of TPP-EMB and HG groups compared to those of the Thi-EMB and EMB groups, and total glutathione (tGSH) was also found to be higher. In addition, severe retinal tissue vascularization, edema and loss of ganglion cells were observed in the Thi-EMB and EMB groups, whereas histopathological findings for the TPP-EMB group were observed to be close to normal. DISCUSSION AND CONCLUSION:
|
Authors | Emine Cinici, Nihal Cetin, Ibrahim Ahiskali, Bahadir Suleyman, Durdu Altuner, Hamit Hakan Alp, Ebru Sener, Ilknur Calik, Halis Suleyman |
Journal | Cutaneous and ocular toxicology
(Cutan Ocul Toxicol)
Vol. 35
Issue 3
Pg. 222-7
(Sep 2016)
ISSN: 1556-9535 [Electronic] England |
PMID | 26339826
(Publication Type: Journal Article)
|
Chemical References |
- Antioxidants
- Antitubercular Agents
- Malondialdehyde
- 8-hydroxyguanine
- Guanine
- Ethambutol
- Glutathione
- Thiamine Pyrophosphate
- Thiamine
|
Topics |
- Animals
- Antioxidants
(pharmacology, therapeutic use)
- Antitubercular Agents
(adverse effects)
- DNA Damage
- Ethambutol
(adverse effects)
- Eye
(drug effects, metabolism, pathology)
- Eye Diseases
(chemically induced, drug therapy, metabolism, pathology)
- Glutathione
(metabolism)
- Guanine
(analogs & derivatives, metabolism)
- Male
- Malondialdehyde
(metabolism)
- Oxidative Stress
(drug effects)
- Rats, Wistar
- Thiamine
(pharmacology, therapeutic use)
- Thiamine Pyrophosphate
(pharmacology, therapeutic use)
|