Mechano growth factor-E regulates apoptosis and inflammatory responses in fibroblast-like synoviocytes of knee osteoarthritis.

Abstract

PURPOSES:

This study investigated whether mechano growth factor-E (MGF-E) peptide can regulate apoptosis and inflammation responses in fibroblast-like synoviocytes of osteoarthritis (OA).

METHODS:

A (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) (MTS) assay was performed to evaluate cytotoxic effects of exogenous MGF-E peptide on OA fibroblast-like synoviocytes (OA-FLS). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to check messenger RNA (mRNA) expression levels of lysyl oxidase (LOX) family members (LOX) after OA-FLS treatment using MGF-E peptide. A 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay was performed to identify the influence of MGF-E peptide on proliferation OA-FLS proliferation. Western blot was used to detect biomarkers of endoplasmic reticulum (ER) stress and inflammatory cytokines.

RESULTS:

Exogenous MGF-E peptide has no obvious cytotoxic effects on OA-FLS and promotes LOX expression in OA-FLS, induce apoptosis and ER stress and down-regulate protein levels of tumour necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β).

CONCLUSIONS:

Our results suggest that MGF-E peptide possesses potential anti-inflammatory effects, induces cell apoptosis and facilitates repair of OA-FLS. Therefore, MGF-E peptide may have therapeutic potential in patients with OA.

• Authors: Haibin Li, Mingxing Lei, Can Yu, Yonggang Lv, Yang Song, Li Yang
• Journal: International orthopaedics (Int Orthop) Vol. 39 Issue 12 Pg. 2503-9 (Dec 2015) ISSN: 1432-5195 [Electronic] Germany
• PMID: 26338342 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Tetrazolium Salts
• Thiazoles
• mechano-growth factor E, human
• 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
• Insulin-Like Growth Factor I
• Protein-Lysine 6-Oxidase
• 5-ethynyl-2'-deoxyuridine
• Deoxyuridine

Topics

• Apoptosis (drug effects)
• Blotting, Western
• Cytokines (metabolism)
• Deoxyuridine (analogs & derivatives)
• Endoplasmic Reticulum Stress (drug effects, physiology)
• Female
• Fibroblasts (drug effects)
• Humans
• Inflammation (metabolism)
• Insulin-Like Growth Factor I (pharmacology)
• Middle Aged
• Osteoarthritis, Knee (metabolism, pathology)
• Protein-Lysine 6-Oxidase (metabolism)
• Real-Time Polymerase Chain Reaction
• Synovial Membrane (cytology, metabolism, pathology)
• Tetrazolium Salts
• Thiazoles