The formation of complete supported
lipid bilayers by vesicle adsorption and
rupture was studied in relation to deposition conditions of vesicles and underlying cushion formed from various
polyelectrolytes.
Lipid vesicles were formed from zwitterionic
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and negatively charged
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) in
phosphate buffer of various pH with or without NaCl addition.
Polyelectrolyte multilayer films (PEM) were constructed by sequential adsorption of alternately charged
polyelectrolytes from their solutions-layer-by-layer deposition (LBL). The mechanism of the formation of supported
lipid bilayer on
polyelectrolyte films was studied by
quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM). QCM-D allowed following the adsorption kinetics while AFM measurements verified the morphology of
lipid vesicles and isolated bilayer patches on the PEM cushions providing local topological images in terms of lateral organization. Additionally,
polyelectrolyte cushions were characterized with ellipsometry to find thickness and swelling properties, and their roughness was determined using AFM. It has been demonstrated that the pH value and an addition of NaCl in the
buffer solution as well as the type of the
polyelectrolyte cushion influence the kinetics of bilayer formation and the quality of formed bilayer patches.