Abstract | OBJECTIVES:
Polyphenols (PP) have demonstrated beneficial effects on low-grade inflammation and oxidative stress; however, little is known about their effect on highly inflamed muscle. The purposes of this study were (i) to evaluate muscle alteration induced by high-grade inflammation, and (ii) to test the effects of red grape PP supplementation on these alterations. METHODS: We used a transgenic mice model ( transforming growth factor [TGF] mice) to develop a high T cell-dependent inflammation and C57 BL/6 control (CTL) mice model. Skeletal muscles of TGF and CTL mice were investigated for inflammation, atrophy and oxidative stress markers. Isolated mitochondria from hindlimb muscles were used for respiration with pyruvate as substrate and oxidative damages were measured by Western blot. TGF mice were supplemented with a mixture of red grape polyphenols (50 mg/kg/d) for 4 wk. Data were analyzed by one-way analysis of variance (ANOVA) and post hoc Bonferroni's multiple comparison tests. RESULTS: CONCLUSIONS: These observations suggest that nutritional dosages of red grape polyphenols might be beneficial for reducing skeletal muscle atrophy, even in a high-grade inflammation environment.
|
Authors | Karen Lambert, Marjorie Coisy-Quivy, Catherine Bisbal, Pascal Sirvent, Gerald Hugon, Jacques Mercier, Antoine Avignon, Ariane Sultan |
Journal | Nutrition (Burbank, Los Angeles County, Calif.)
(Nutrition)
Vol. 31
Issue 10
Pg. 1275-83
(Oct 2015)
ISSN: 1873-1244 [Electronic] United States |
PMID | 26333892
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
|
Topics |
- Analysis of Variance
- Animals
- Caspases
(metabolism)
- Dietary Supplements
- Hindlimb
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Mitochondria, Muscle
(metabolism)
- Models, Animal
- Muscle, Skeletal
(drug effects, immunology)
- Muscular Atrophy
(diet therapy)
- Myositis
(diet therapy)
- Oxidative Stress
(immunology)
- Polyphenols
(administration & dosage)
- Signal Transduction
(drug effects)
- Vitis
(chemistry)
|