Effects of High-Intensity Swimming on Lung Inflammation and Oxidative Stress in a Murine Model of DEP-Induced Injury.

Abstract

Studies have reported that exposure to diesel exhaust particles (DEPs) induces lung inflammation and increases oxidative stress, and both effects are susceptible to changes via regular aerobic exercise in rehabilitation programs. However, the effects of exercise on lungs exposed to DEP after the cessation of exercise are not clear. Therefore, the aim of this study was to evaluate the effects of high-intensity swimming on lung inflammation and oxidative stress in mice exposed to DEP concomitantly and after exercise cessation. Male Swiss mice were divided into 4 groups: Control (n = 12), Swimming (30 min/day) (n = 8), DEP (3 mg/mL-10 μL/mouse) (n = 9) and DEP+Swimming (n = 8). The high-intensity swimming was characterized by an increase in blood lactate levels greater than 1 mmoL/L between 10th and 30th minutes of exercise. Twenty-four hours after the final exposure to DEP, the anesthetized mice were euthanized, and we counted the number of total and differential inflammatory cells in the bronchoalveolar fluid (BALF), measured the lung homogenate levels of IL-1β, TNF-α, IL-6, INF-ϫ, IL-10, and IL-1ra using ELISA, and measured the levels of glutathione, non-protein thiols (GSH-t and NPSH) and the antioxidant enzymes catalase and glutathione peroxidase (GPx) in the lung. Swimming sessions decreased the number of total cells (p<0.001), neutrophils and lymphocytes (p<0.001; p<0.05) in the BALF, as well as lung levels of IL-1β (p = 0.002), TNF-α (p = 0.003), IL-6 (p = 0.0001) and IFN-ϫ (p = 0.0001). However, the levels of IL-10 (p = 0.01) and IL-1ra (p = 0.0002) increased in the swimming groups compared with the control groups, as did the CAT lung levels (p = 0.0001). Simultaneously, swimming resulted in an increase in the GSH-t and NPSH lung levels in the DEP group (p = 0.0001 and p<0.002). We concluded that in this experimental model, the high-intensity swimming sessions decreased the lung inflammation and oxidative stress status during DEP-induced lung inflammation in mice.

• Authors: Leonardo C M Ávila, Thayse R Bruggemann, Franciane Bobinski, Morgana Duarte da Silva, Regiane Carvalho Oliveira, Daniel Fernandes Martins, Leidiane Mazzardo-Martins, Marta Maria Medeiros Frescura Duarte, Luiz Felipe de Souza, Alcir Dafre, Rodolfo de Paula Vieira, Adair Roberto Soares Santos, Kelly Cattelan Bonorino, Deborah de C Hizume Kunzler
• Journal: PloS one (PLoS One) Vol. 10 Issue 9 Pg. e0137273 ( 2015) ISSN: 1932-6203 [Electronic] United States
• PMID: 26332044 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Inflammation Mediators
• Sulfhydryl Compounds
• Vehicle Emissions
• Catalase
• Glutathione

Topics

• Animals
• Bronchoalveolar Lavage Fluid
• Catalase (metabolism)
• Cytokines (metabolism)
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Glutathione (metabolism)
• Inflammation Mediators (metabolism)
• Lung (drug effects, enzymology, metabolism)
• Male
• Mice
• Oxidative Stress
• Pneumonia (chemically induced, physiopathology)
• Sulfhydryl Compounds (metabolism)
• Swimming
• Vehicle Emissions (toxicity)