Urinary 5-hydroxymethyluracil and 8-oxo-7,8-dihydroguanine as potential biomarkers in patients with colorectal cancer.

Abstract

CONTEXT:

Oxidative stress linked with chronic inflammation is associated with etiology of the colorectal cancer.

OBJECTIVES:

To assess the diagnostic utility of urinary excretion of oxidatively modified DNA bases/nucleoside: 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 5-hydroxymethyluracil (5-hmUra).

MATERIALS AND METHODS:

Seventy-two healthy controls, 15 patients with adenomas and 56 colorectal cancer patients were recruited.

RESULTS:

The receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) for all markers tested separately was <0.7. The combination of these modifications showed better diagnostic power (AUC = 0.778 for 8-oxoGua + 8-oxodG)/5hmUra ratio).

CONCLUSION:

Urinary DNA modifications may reflect the oxidative stress/chronic inflammation in colorectal cancer but diagnostic performance for early-detection is moderate.

• Authors: Rafal Rozalski, Daniel Gackowski, Agnieszka Siomek-Gorecka, Marta Starczak, Martyna Modrzejewska, Zbigniew Banaszkiewicz, Ryszard Olinski
• Journal: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals (Biomarkers) Vol. 20 Issue 5 Pg. 287-91 ( 2015) ISSN: 1366-5804 [Electronic] England
• PMID: 26329524 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• 5-hydroxymethyluracil
• Guanine
• 8-oxo-7,8-dihydrodeoxyguanine
• Pentoxyl
• 8-Hydroxy-2'-Deoxyguanosine

Topics

• 8-Hydroxy-2'-Deoxyguanosine (analogs & derivatives)
• Aged
• Biomarkers, Tumor (urine)
• Colorectal Neoplasms (urine)
• Female
• Guanine (analogs & derivatives, urine)
• Humans
• Male
• Middle Aged
• Pentoxyl (analogs & derivatives, urine)