Abstract |
Consumption of antioxidant-enriched diets is 1 method of addressing obesity, which is associated with chronic oxidative stress and changes in the activity/expression of various enzymes. In this study, we hypothesized that the modulation of antioxidant enzymes and redox status through a cranberry extract (CBE)-enriched diet would differ between obese and nonobese mice. The CBE used in this study was obtained from the American cranberry (Vaccinium macrocarpon, Ericaceae), a popular constituent of dietary supplements that is a particularly rich source of (poly) phenols and has strong antioxidant properties. The present study was designed to test and compare the in vivo effects of 28-day consumption of a CBE-enriched diet (2%) on the antioxidant status of nonobese mice and mice with monosodium glutamate-induced obesity. Plasma, erythrocytes, liver, and small intestine were studied concurrently to obtain more complex information. The specific activities, protein, and messenger RNA expression levels of antioxidant enzymes as well as the levels of malondialdehyde and thiol (SH) groups were analyzed. Cranberry extract treatment increased the SH group content in plasma and the glutathione S-transferase activity in the erythrocytes of the obese and nonobese mice. In addition, in the obese animals, the CBE treatment reduced the malondialdehyde content in erythrocytes and increased NAD(P)H: NAD(P)H:
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Authors | Iva Boušová, Hana Bártíková, Petra Matoušková, Kateřina Lněničková, Lukáš Zappe, Kateřina Valentová, Barbora Szotáková, Jan Martin, Lenka Skálová |
Journal | Nutrition research (New York, N.Y.)
(Nutr Res)
Vol. 35
Issue 10
Pg. 901-909
(Oct 2015)
ISSN: 1879-0739 [Electronic] United States |
PMID | 26319613
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
- Antioxidants
- Plant Extracts
- RNA, Messenger
- Sulfhydryl Compounds
- Malondialdehyde
- Catalase
- NAD(P)H Dehydrogenase (Quinone)
- Noq1 protein, mouse
- Glutathione Transferase
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Topics |
- Animals
- Antioxidants
(administration & dosage)
- Catalase
(blood, metabolism)
- Diet
- Erythrocytes
(chemistry)
- Fruit
(chemistry)
- Glutathione Transferase
(blood)
- Intestine, Small
(enzymology)
- Liver
(enzymology)
- Malondialdehyde
(blood)
- Mice
- NAD(P)H Dehydrogenase (Quinone)
(genetics, metabolism)
- Obesity
(blood, chemically induced, enzymology)
- Oxidation-Reduction
- Plant Extracts
(administration & dosage)
- RNA, Messenger
(analysis)
- Sulfhydryl Compounds
(blood)
- Vaccinium macrocarpon
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