The use of in vitro assays for neoplastic transformation and tumor promotion.

Abstract

We investigated growth control in mixed cultures of normal and oncogene-transformed mouse fibroblasts. NIH/3T3 transformed by v-myc, polyoma large T, polyoma middle T, v-ras and v-src showed comparable cloning efficiencies in agarized medium. However, when cultivated with an excess of normal cells (Balb/3T3, C3H10T1/2 or primary rat and hamster embryo cells) ras, src, and middle T-transformed cells were able to form "foci" of transformation on the layer of density arrested normal cells, whereas myc- and polyoma large T-transformed cells lacked this ability. Addition of the phorbol ester tumor promoter, phorbol-12,13-didecanoate, rescued proliferation and focus-formation by these nuclear oncogenes-transformed cell lines. Evidence is presented and discussed supporting a main role of intercellular communication between normal and transformed cells in modulating suppression or expression of the transformed phenotype.

• Authors: S Rosa, M Bignami
• Journal: Annali dell'Istituto superiore di sanita (Ann Ist Super Sanita) Vol. 25 Issue 4 Pg. 545-8 ( 1989) ISSN: 0021-2571 [Print] Italy
• PMID: 2631620 (Publication Type: Journal Article)

Chemical References

• Carcinogens
• Phorbol Esters
• phorbol-12,13-didecanoate

Topics

• Animals
• Carcinogens (pharmacology)
• Cell Line
• Cell Transformation, Neoplastic (analysis, drug effects)
• Cricetinae
• Fibroblasts (cytology)
• Oncogenes (physiology)
• Phorbol Esters (pharmacology)
• Rats