Abstract | OBJECTIVE: We previously showed that the cerebellum modulates the immune system. Here we determined whether cerebellar ataxia alters immunological function to further demonstrate an involvement of the cerebellum in immune modulation. METHODS:
Neurotoxin 3-acetylpyridine (3-AP) was intraperitoneally injected in rats to induce cerebellar ataxia. Behavior and motor coordination were tested on day 7 following 3-AP injection. Nissl staining and high-performance liquid chromatography (HPLC) were used to determine neuronal loss and neurotransmitter contents, respectively, in all the three cerebellar nuclei, fastigial nucleus (FN), interposed nucleus (IN) and dentate nucleus (DN). T and B lymphocyte differentiation and function were measured by flow cytometry, Western blot and ELISA. RESULTS: 3-AP induced motor discoordination and locomotor reduction. In all the three cerebellar nuclei, FN, IN and DN, there was a neuronal loss and a decrease in contents of glutamate and GABA (but not glycine) after 3-AP injection. Importantly, CD4+ T cells, but not CD8+ T cells, were increased by the 3-AP treatment. Moreover, interferon (IFN)-γ-producing cells and interleukin (IL)-17-producing cells were decreased in cerebellar ataxia rats, but IL-4-producing cells and CD25-expressing cells were increased. Expression of the T helper (Th)1- and Th17-related cytokines, IFN-γ, IL-2, IL-17 and IL-22, was downregulated in CD4+ cells in cerebellar ataxia rats, while expression of the Th2 and regulatory T (Treg)-related cytokines, IL-4, IL-5, IL-10 and transforming growth factor (TGF)-β, was upregulated. Furthermore, B lymphocyte number and anti- bovine serum albumin (BSA) IgM and IgG antibody levels were elevated in cerebellar ataxia. CONCLUSION:
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Authors | Yong-Ying Jiang, Bei-Bei Cao, Xiao-Qin Wang, Yu-Ping Peng, Yi-Hua Qiu |
Journal | Neuro endocrinology letters
(Neuro Endocrinol Lett)
Vol. 36
Issue 3
Pg. 246-56
( 2015)
ISSN: 0172-780X [Print] Sweden |
PMID | 26313392
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Cerebellar Ataxia
(chemically induced, immunology, metabolism)
- Disease Models, Animal
- Immunity, Cellular
(immunology)
- Immunity, Humoral
(immunology)
- Male
- Rats
- Rats, Sprague-Dawley
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