Abstract | OBJECTIVES/HYPOTHESIS: STUDY DESIGN: Prospective, randomized, controlled animal study. METHODS: A crush-type facial nerve injury was performed on the right side of all rats (injury group [IG]), whereas there was no injury on the left side ( sham group [SG]). These main groups were divided into five subgroups: 1) no medicine (control); 2) physiological serum; 3) dexamethasone; 4) oxytocin; and 5) resveratrol (Res) administered ( intraperitoneal injection) for 28 days. Functional recovery was evaluated by daily eye-blink reflex and facial electromyography. Nerve-muscle degeneration and regeneration, apoptosis, and intercellular connections were evaluated in histopathological and immunohistochemical examinations. RESULTS: Recovery time of the postinjury eye-blink reflex demonstrated faster recovery in IG+Res when compared with the other subgroups. In peak-to-peak amplitude values, a significant increase was observed in the dexamethasone (P=0.007) and oxytocin subgroups (P=0.004) and was even more apparent in the resveratrol subgroup (P<0.001). Nerve regeneration is apparent in the resveratrol subgroup. Apoptotic changes were evaluated immunohistochemically with TUNEL and Caspase 3 and 6 antibodies staining. Caspase 3 and 6 immunoexpressions of resveratrol and oxytocin subgroups were moderate when compared with dexamethasone subgroup. Except for the resveratrol subgroup, which had an increase in expression, the majority of subgroups were similar to SG in terms of intercellular connections ( Connexin 32 and 43). CONCLUSION: LEVEL OF EVIDENCE: N/A.
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Authors | Gokce Tanyeri, Onur Celik, Oytun Erbas, Fatih Oltulu, Ozlem Yilmaz Dilsiz |
Journal | The Laryngoscope
(Laryngoscope)
Vol. 125
Issue 11
Pg. E356-64
(Nov 2015)
ISSN: 1531-4995 [Electronic] United States |
PMID | 26308241
(Publication Type: Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 The American Laryngological, Rhinological and Otological Society, Inc. |
Chemical References |
- Neuroprotective Agents
- Stilbenes
- Oxytocin
- Dexamethasone
- Caspase 3
- Caspase 6
- Resveratrol
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Topics |
- Animals
- Caspase 3
(analysis)
- Caspase 6
(analysis)
- Dexamethasone
(therapeutic use)
- Electrophysiology
- Facial Nerve Injuries
(drug therapy, pathology, physiopathology)
- Immunohistochemistry
- In Situ Nick-End Labeling
- Nerve Regeneration
(drug effects)
- Neuroprotective Agents
(therapeutic use)
- Oxytocin
(therapeutic use)
- Prospective Studies
- Rats
- Rats, Sprague-Dawley
- Resveratrol
- Stilbenes
(therapeutic use)
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