Ameliorative effect of vanadyl(IV)-ascorbate complex on high-fat high-sucrose diet-induced hyperglycemia, insulin resistance, and oxidative stress in mice.

Abstract

There is mounting evidence demonstrating causative links between hyperglycemia, oxidative stress, and insulin resistance, the core pathophysiological features of type 2 diabetes mellitus. Using a combinational approach, we synthesized a vanadium-antioxidant (i.e., l-ascorbic acid) complex and examined its effect on insulin resistance and oxidative stress. This study was designed to examine whether vanadyl(IV)-ascorbate complex (VOAsc) would reduce oxidative stress, hyperglycemia, and insulin resistance in high-fat high-sucrose diet (HFSD)-induced type 2 diabetes in mice. Male C57BL/6J mice were fed a HFSD for 12 weeks to induce insulin resistance, rendering them diabetic. Diabetic mice were treated with rosiglitazone, sodium l-ascorbate, or VOAsc. At the end of treatment, fasting blood glucose, fasting serum insulin, homeostasis model assessment-insulin resistance index, and serum adipocytokine levels were measured. Serum levels of nitric oxide (NO) parameters were also determined. The liver was isolated and used for determination of malondialdehyde, reduced glutathione, and catalase levels, and superoxide dismutase and glutathione peroxidase activities. VOAsc groups exhibited significant reductions in serum adipocytokine and NO levels, and oxidative stress parameters compared to the corresponding values in the untreated diabetic mice. The results indicated that VOAsc is non-toxic. In conclusion, we identified VOAsc as a potentially effective adjunct therapy for the management of type 2 diabetes.

• Authors: Yanjun Liu, Jie Xu, Yongli Guo, Yong Xue, Jingfeng Wang, Changhu Xue
• Journal: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) (J Trace Elem Med Biol) Vol. 32 Pg. 155-61 (Oct 2015) ISSN: 1878-3252 [Electronic] Germany
• PMID: 26302923 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier GmbH. All rights reserved.

Chemical References

• Adiponectin
• Blood Glucose
• Insulin
• Leptin
• Resistin
• Tumor Necrosis Factor-alpha
• Nitric Oxide
• Vanadates
• Malondialdehyde
• Sucrose
• Ascorbic Acid

Topics

• Adiponectin (blood)
• Adipose Tissue (drug effects)
• Animals
• Ascorbic Acid (pharmacology, therapeutic use)
• Blood Glucose (metabolism)
• Body Weight (drug effects)
• Diabetes Mellitus, Type 2 (blood, drug therapy, enzymology)
• Diet, High-Fat
• Feeding Behavior
• Glucose Tolerance Test
• Homeostasis (drug effects)
• Hyperglycemia (blood, complications, drug therapy)
• Insulin (blood)
• Insulin Resistance
• Leptin (blood)
• Liver (drug effects, enzymology)
• Male
• Malondialdehyde (metabolism)
• Mice, Inbred C57BL
• Nitric Oxide (blood)
• Organ Size (drug effects)
• Oxidative Stress (drug effects)
• Resistin (blood)
• Sucrose
• Tumor Necrosis Factor-alpha (blood)
• Vanadates (pharmacology, therapeutic use)