Comparative analyses of genotoxicity, oxidative stress and antioxidative defence system under exposure of methyl parathion and hexaconazole in barley (Hordeum vulgare L.).

Abstract

The present study aims to evaluate the comparative effects of methyl parathion and hexaconazole on genotoxicity, oxidative stress, antioxidative defence system and photosynthetic pigments in barley (Hordeum vulgare L. variety karan-16). The seeds were exposed with three different concentrations, i.e. 0.05, 0.1 and 0.5 % for 6 h after three pre-soaking durations 7, 17 and 27 h which represents G1, S and G2 phases of the cell cycle, respectively. Ethyl methane sulphonate, a well-known mutagenic agent and double distilled water, was used as positive and negative controls, respectively. The results indicate significant decrease in mitotic index with increasing concentrations of pesticides, and the extent was higher in methyl parathion. Chromosomal aberrations were found more frequent in methyl parathion than hexaconazole as compared to their respective controls. Treatment with the pesticides induced oxidative stress which was evident with higher contents of H2O2 and lipid peroxidation, and the increase was more prominent in methyl parathion. Contents of total phenolics were increased; however, soluble protein content showed a reverse trend. Among the enzymatic antioxidants, activities of superoxide dismutase and peroxidase were significantly up-regulated, and more increase was noticed in hexaconazole. Increments in total chlorophyll and carotenoid contents were observed up to 0.1 % but decreased at higher concentration (0.5 %), and the reductions were more prominent in methyl parathion than hexaconazole as compared to their respective controls. Methyl parathion treatment caused more damage in the plant cells of barley as compared to hexaconazole, which may be closely related to higher genotoxicity and oxidative stress.

• Authors: Pragyan Dubey, Amit Kumar Mishra, Ashok Kumar Singh
• Journal: Environmental science and pollution research international (Environ Sci Pollut Res Int) Vol. 22 Issue 24 Pg. 19848-59 (Dec 2015) ISSN: 1614-7499 [Electronic] Germany
• PMID: 26286802 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Mutagens
• Pesticides
• Triazoles
• Chlorophyll
• Methyl Parathion
• Hydrogen Peroxide
• Peroxidases
• Superoxide Dismutase
• hexaconazole

Topics

• Antioxidants (metabolism)
• Cell Cycle (drug effects, genetics)
• Chlorophyll (metabolism)
• DNA Damage
• Hordeum (cytology, drug effects, genetics, metabolism)
• Hydrogen Peroxide (metabolism)
• Lipid Peroxidation (drug effects, genetics)
• Methyl Parathion (toxicity)
• Mutagenicity Tests
• Mutagens (toxicity)
• Oxidative Stress (drug effects)
• Peroxidases (metabolism)
• Pesticides (toxicity)
• Photosynthesis (drug effects, genetics)
• Superoxide Dismutase (metabolism)
• Triazoles (toxicity)