HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Rapid and Quantitative Detection of Avian Influenza A(H7N9) Virions in Complex Matrices Based on Combined Magnetic Capture and Quantum Dot Labeling.

Abstract
Avian influenza A(H7N9) virus, which emerged in China in the spring of 2013, has infected hundreds of people and resulted in many deaths. Herein, a rapid and quantitative assay is proposed for the one-step detection of H7N9 virions. Immunomagnetic nanospheres (IMNs) and antibody-conjugated quantum dots (Ab-QDs) are simultaneously employed to capture and identify the target virus, leading to a high efficiency, good specificity, and strong anti-interference ability. Moreover, this reliable detection assay, which combines the efficient magnetic enrichment and the unique photophysical properties of QDs, can achieve a high sensitivity for a low detection limit. At the same time, this detection strategy shows great flexibility for employment in a variety of fluorescence detectors, including fluorescence spectrometry, microscope assays, and handheld UV lamp tests. Furthermore, our one-step detection strategy induces very little change in the integrity of the vulnerable virions, which enables additional genotyping testing following the fluorescence detection. The present study, thus, reports a rapid and quantitative approach for the detection of H7N9 virions based on simultaneous magnetic capture and QD labeling, thereby providing a higher probability for detection and therefore faster diagnosis of H7N9-infected patients.
AuthorsMin Wu, Zhi-Ling Zhang, Gang Chen, Cong-Ying Wen, Ling-Ling Wu, Jiao Hu, Chao-Chao Xiong, Jian-Jun Chen, Dai-Wen Pang
JournalSmall (Weinheim an der Bergstrasse, Germany) (Small) Vol. 11 Issue 39 Pg. 5280-8 (Oct 21 2015) ISSN: 1613-6829 [Electronic] Germany
PMID26280101 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Topics
  • Immunomagnetic Separation (methods)
  • Influenza A Virus, H7N9 Subtype (isolation & purification, ultrastructure)
  • Microscopy, Fluorescence (methods)
  • Quantum Dots
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Staining and Labeling (methods)
  • Viral Load (methods)
  • Virion (isolation & purification, ultrastructure)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: