Abstract |
Avian influenza A(H7N9) virus, which emerged in China in the spring of 2013, has infected hundreds of people and resulted in many deaths. Herein, a rapid and quantitative assay is proposed for the one-step detection of H7N9 virions. Immunomagnetic nanospheres (IMNs) and antibody-conjugated quantum dots (Ab-QDs) are simultaneously employed to capture and identify the target virus, leading to a high efficiency, good specificity, and strong anti-interference ability. Moreover, this reliable detection assay, which combines the efficient magnetic enrichment and the unique photophysical properties of QDs, can achieve a high sensitivity for a low detection limit. At the same time, this detection strategy shows great flexibility for employment in a variety of fluorescence detectors, including fluorescence spectrometry, microscope assays, and handheld UV lamp tests. Furthermore, our one-step detection strategy induces very little change in the integrity of the vulnerable virions, which enables additional genotyping testing following the fluorescence detection. The present study, thus, reports a rapid and quantitative approach for the detection of H7N9 virions based on simultaneous magnetic capture and QD labeling, thereby providing a higher probability for detection and therefore faster diagnosis of H7N9-infected patients.
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Authors | Min Wu, Zhi-Ling Zhang, Gang Chen, Cong-Ying Wen, Ling-Ling Wu, Jiao Hu, Chao-Chao Xiong, Jian-Jun Chen, Dai-Wen Pang |
Journal | Small (Weinheim an der Bergstrasse, Germany)
(Small)
Vol. 11
Issue 39
Pg. 5280-8
(Oct 21 2015)
ISSN: 1613-6829 [Electronic] Germany |
PMID | 26280101
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Topics |
- Immunomagnetic Separation
(methods)
- Influenza A Virus, H7N9 Subtype
(isolation & purification, ultrastructure)
- Microscopy, Fluorescence
(methods)
- Quantum Dots
- Reproducibility of Results
- Sensitivity and Specificity
- Staining and Labeling
(methods)
- Viral Load
(methods)
- Virion
(isolation & purification, ultrastructure)
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