Abstract |
During maturation to an erythrocyte, a reticulocyte must eliminate any residual organelles and reduce its surface area and volume. Here we show this involves a novel process whereby large, intact, inside-out phosphatidylserine (PS)-exposed autophagic vesicles are extruded. Cell surface PS is a well-characterized apoptotic signal initiating phagocytosis. In peripheral blood from patients after splenectomy or in patients with sickle cell disease (SCD), the number of circulating red cells exposing PS on their surface is elevated. We show that in these patients PS is present on the cell surface of red cells in large (∼1.4 µm) discrete areas corresponding to autophagic vesicles. The autophagic vesicles found on reticulocytes are identical to those observed on red cells from splenectomized individuals and patients with SCD. Our data suggest the increased thrombotic risk associated with splenectomy, and patients with hemoglobinopathies is a possible consequence of increased levels of circulating mature reticulocytes expressing inside-out PS-exposed autophagic vesicles because of asplenia.
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Authors | Tosti J Mankelow, Rebecca E Griffiths, Sara Trompeter, Joanna F Flatt, Nicola M Cogan, Edwin J Massey, David J Anstee |
Journal | Blood
(Blood)
Vol. 126
Issue 15
Pg. 1831-4
(Oct 08 2015)
ISSN: 1528-0020 [Electronic] United States |
PMID | 26276668
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 by The American Society of Hematology. |
Chemical References |
- Glycophorins
- Phosphatidylserines
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Topics |
- Anemia, Sickle Cell
(blood, pathology)
- Autophagy
- Blotting, Western
- Case-Control Studies
- Cell Proliferation
- Cells, Cultured
- Erythrocytes
(metabolism, pathology)
- Flow Cytometry
- Glycophorins
(metabolism)
- Humans
- Image Processing, Computer-Assisted
- Phagocytosis
- Phosphatidylserines
(chemistry, metabolism)
- Reticulocytes
(metabolism, pathology)
- Splenectomy
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