Abstract | BACKGROUND: METHODS: RESULTS: A novel cell line, designed as Thy0517, was established from thymoma type AB with myasthenia gravis patients by tissue explant. As an immortalized cell line, it always has a stable growth cycle, and there is no change in characteristics and morphology after culturing for 18 months and passing 160 generations in vitro. The experimental data demonstrate that the cell line exhibits the growth characteristics of tumor cells, the doubling time of 37 hours, with tumorigenicity in vitro and chromosome abnormality. Immunocytochemistry indicated that the cell line positive expression of CK7, CK8/18, CK19, CK-pan, CD24, BCL-2, P63, Vimentin, epithelial membrane antigen and epidermal growth factor receptor, lymphocyte related antigen CD99, and TdT were negatively expressed. CONCLUSIONS:
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Authors | Guojin Wang, Yuanguo Wang, Peng Zhang, Yuan Chen, Yimei Liu, Feng Guo, Hui Zhang |
Journal | Thoracic cancer
(Thorac Cancer)
Vol. 6
Issue 2
Pg. 194-201
(Mar 2015)
ISSN: 1759-7706 [Print] Singapore |
PMID | 26273358
(Publication Type: Journal Article)
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