δ-Amyrone inhibits lipopolysaccharide-induced inflammatory cytokines and protects against endotoxic shock in mice.

Abstract

δ-Amyrone (13(18)-Oleanen-3-one), which is an active constituent extracted and separated from Sedum lineare Thunb., has been found to possess a potent anti-inflammatory effect in different inflammation model animals. But its effects on lipopolysaccharide (LPS)-induced endotoxic shock have not been previous explored. The aim of this study is to evaluate the effect of δ-Amyrone on LPS-induced inflammatory cytokines and the protective effect on endotoxic shock mice. Experimental animals received δ-amyrone (4 and 8 mg/kg, i.p.) and dexamethasone (DEX) (5 mg/kg, i.p.) at 24 and 1 h before LPS injection. δ-Amyrone treatment significantly decreased mortality rate, tissues myeloperoxodase (MPO) activity, p65 NF-κB protein expression when compared with the LPS groups. The levels of tumor nectosis factor-alphagene (TNF-α) and interleukin-6 (IL-6) both in serum and lung, liver, kidney tissues, as well as the accumulation of nitric oxide (NO) in serum were decreased by δ-amyrone in response to p65 nuclear factors-kappa B (NF-κB). These results suggest that the protective activity of δ-amyrone on LPS-induced endotoxic shock is attributed to reducing NO production and mediating the pro-inflammatory cytokines, inhibited NF-κB expression.

• Authors: Xiaofeng Niu, Huan Yao, Weifeng Li, Qingli Mu, Huani Li, Yu Wang, Hailin Zhang
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 240 Pg. 354-61 (Oct 05 2015) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 26271896 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Cytokines
• Interleukin-6
• Lipopolysaccharides
• NF-kappa B
• Protective Agents
• Triterpenes
• Tumor Necrosis Factor-alpha
• amyrone
• Peroxidase

Topics

• Animals
• Cytokines (antagonists & inhibitors, metabolism)
• Gene Expression Regulation, Enzymologic (drug effects)
• Immunohistochemistry
• Inflammation (chemically induced)
• Interleukin-6 (blood)
• Kidney (enzymology)
• Lipopolysaccharides
• Liver (enzymology)
• Lung (enzymology)
• Male
• Mice
• NF-kappa B (blood)
• Peroxidase (chemistry)
• Protective Agents (pharmacology)
• Shock, Septic (prevention & control)
• Triterpenes (pharmacology)
• Tumor Necrosis Factor-alpha (blood)