Visualizing myocardial inflammation in a rat model of type 4 cardiorenal syndrome by dual-modality molecular imaging.

Abstract

Type 4 cardiorenal syndrome (CRS) is a life-threatening world health problem in which chronic kidney disease leads to progressive cardiovascular disease. In type 4 CRS, cardiac inflammation is an excellent target for both detection and therapy; however, this progression was underestimated by previous studies due to the lack of effective detection methods. To noninvasively visualize cardiac inflammation and monitor therapeutic efficacy of anti-inflammatory treatment in type 4 CRS, we here synthesized a dual-modality magneto-fluorescent nanoparticle (MNP) by combining ultrasmall superparamagnetic iron oxide nanoparticle and Rhodamine B for both magnetic resonance imaging (MRI) and optical imaging. This dual-functional MNP exhibited excellent performance such as high r2 relaxivity coefficient (283.4 mM(-1) s(-1)), high magnetism (96.7 emu/g iron) and a near neutral surface charge to minimize the reticuloendothelial system uptake. In vivo cardiac MRI showed significant negative contrast in the type 4 CRS rats, and the signal intensity on optical imaging was significantly higher in the type 4 CRS group compared with sham-operated and drug-treated groups. The specific targeting profile of MNPs to monocyte-macrophages was proven by histopathological analysis. Taken together, we demonstrate that this dual-modality strategy is feasible for noninvasively assessing myocardial inflammation and monitoring therapeutic efficacy in type 4 CRS.

• Authors: Di Chang, Yuan-Cheng Wang, Shi-Jun Zhang, Ying-Ying Bai, Dong-Fang Liu, Feng-Chao Zang, Guozheng Wang, Binghui Wang, Shenghong Ju
• Journal: Biomaterials (Biomaterials) Vol. 68 Pg. 67-76 (Nov 2015) ISSN: 1878-5905 [Electronic] Netherlands
• PMID: 26264647 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Contrast Media
• Dextrans
• Magnetite Nanoparticles
• Nanocapsules
• Rhodamines
• ferumoxtran-10
• rhodamine B

Topics

• Animals
• Cardio-Renal Syndrome (pathology)
• Contrast Media
• Dextrans (chemistry)
• Magnetic Resonance Imaging (methods)
• Magnetite Nanoparticles (chemistry)
• Male
• Microscopy, Fluorescence (methods)
• Multimodal Imaging (methods)
• Myocarditis (diagnosis, pathology)
• Nanocapsules (chemistry, ultrastructure)
• Rats
• Rats, Sprague-Dawley
• Reproducibility of Results
• Rhodamines (chemistry)
• Sensitivity and Specificity