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Retinoblastoma-binding protein 2 induces epithelial-mesenchymal transition in esophageal squamous cancer cells.

AbstractOBJECTIVES:
To investigate the effect of retinoblastoma-binding protein 2 (RBP2) on epithelial-mesenchymal transition (EMT) in esophageal squamous cancer cells and to compare the effect of RBP2 in lung squamous cancer cells and esophageal squamous cancer cells.
RESULTS:
When transfected with RBP2 siRNA, the migrated cells were 36.3 ± 6.03 by transwell migration assay, compared to 107 ± 6.7 cells in the control group. The mRNA level of epithelial cadherin (E-cadherin) was 1.54 ± 0.14 times higher than in the control group, and that of neural cadherin (N-cadherin) fell to 0.76 ± 0.03 times. The relative luciferase activity of E-cadherin promoter rose to 3.84 ± 0.23 times. Correspondingly, the expression of E-cadherin protein increased and that of N-cadherin protein decreased. When SK-MES-1 cells were transfected with RBP2 siRNA, their relative mRNA level of E-cadherin was 8.6 ± 0.37 times as high as that in control group, which was higher than that in Eca-109 cells. The E-cadherin protein was also greater in SK-MES-1 cells.
CONCLUSION:
RBP2 could induce EMT in esophageal cancer cells and exert a greater effect on the expression of E-cadherin in lung squamous cells than in esophageal squamous cells.
AuthorsNaian Qiao, Shikun Wang, Likuan Hu
JournalBiotechnology letters (Biotechnol Lett) Vol. 37 Issue 12 Pg. 2365-70 (Dec 2015) ISSN: 1573-6776 [Electronic] Netherlands
PMID26264242 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cadherins
  • Retinoblastoma-Binding Protein 2
Topics
  • Cadherins (analysis)
  • Carcinoma, Squamous Cell (pathology)
  • Cell Line, Tumor
  • Epithelial-Mesenchymal Transition
  • Esophageal Neoplasms (pathology)
  • Gene Knockdown Techniques
  • Humans
  • Lung Neoplasms (pathology)
  • Models, Biological
  • Retinoblastoma-Binding Protein 2 (genetics, metabolism)

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