Abstract | OBJECTIVES: RESULTS: When transfected with RBP2 siRNA, the migrated cells were 36.3 ± 6.03 by transwell migration assay, compared to 107 ± 6.7 cells in the control group. The mRNA level of epithelial cadherin ( E-cadherin) was 1.54 ± 0.14 times higher than in the control group, and that of neural cadherin ( N-cadherin) fell to 0.76 ± 0.03 times. The relative luciferase activity of E-cadherin promoter rose to 3.84 ± 0.23 times. Correspondingly, the expression of E-cadherin protein increased and that of N-cadherin protein decreased. When SK-MES-1 cells were transfected with RBP2 siRNA, their relative mRNA level of E-cadherin was 8.6 ± 0.37 times as high as that in control group, which was higher than that in Eca-109 cells. The E-cadherin protein was also greater in SK-MES-1 cells. CONCLUSION: RBP2 could induce EMT in esophageal cancer cells and exert a greater effect on the expression of E-cadherin in lung squamous cells than in esophageal squamous cells.
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Authors | Naian Qiao, Shikun Wang, Likuan Hu |
Journal | Biotechnology letters
(Biotechnol Lett)
Vol. 37
Issue 12
Pg. 2365-70
(Dec 2015)
ISSN: 1573-6776 [Electronic] Netherlands |
PMID | 26264242
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cadherins
- Retinoblastoma-Binding Protein 2
|
Topics |
- Cadherins
(analysis)
- Carcinoma, Squamous Cell
(pathology)
- Cell Line, Tumor
- Epithelial-Mesenchymal Transition
- Esophageal Neoplasms
(pathology)
- Gene Knockdown Techniques
- Humans
- Lung Neoplasms
(pathology)
- Models, Biological
- Retinoblastoma-Binding Protein 2
(genetics, metabolism)
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