Abstract | OBJECTIVE: METHODS: WHO microtest, pLDH, HRP II and SYBR Green I were applied to test the drug efficacy of chloroquine, piperaquine and amodiaquine against four Plasmodiumfalciparum reference strains (3D7, FCC, K1 and Dd2), respectively. The consistency of the 50% inhibitory concentration (IC50) values from the four assay methods were analyzed by Friedman tests, Partial correlation analysis, Pearson' s correlation analysis and Bland-Altman plots. RESULTS: With the initial parasitemia ranged from 0.5% to 1%, there were no statistically significant differences (P> 0.05) among the IC50 values obtained by the four assay methods, which were correlated well (both P< 0.001). WHO microtest was highly labor-intensive, time-consuming and subjective; although HRP II was more sensitive than pLDH and SYBR Green I, which was more expensive; SYBR Green I was a simple, rapid and economic assay method. CONCLUSION:
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Authors | Mei-hua Zhang, Feng Lu, Jun Cao, Qi Gao |
Journal | Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control
(Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi)
Vol. 27
Issue 2
Pg. 146-51
(Apr 2015)
ISSN: 1005-6661 [Print] China |
PMID | 26263774
(Publication Type: Comparative Study, English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Antimalarials
(pharmacology)
- In Vitro Techniques
- Parasitic Sensitivity Tests
(methods)
- Plasmodium falciparum
(drug effects)
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