Novel relationships between B12, folate and markers of inflammation, oxidative stress and NAD(H) levels, systemically and in the CNS of a healthy human cohort.

Abstract

OBJECTIVE:

To evaluate the relationship between folate, cobalamin (Cbl), and homocysteine (Hcy), and markers of inflammation and oxidative stress within the periphery and central nervous system (CNS) of a healthy human cohort.

METHODS:

Thirty-five matched cerebrospinal fluid (CSF) and plasma samples were collected from consenting participants who required a spinal tap for the administration of anaesthetic. Plasma concentrations of Hcy and both plasma and CSF levels of folate, Cbl, nicotinamide adenine dinucleotide (NAD(H)) and markers of inflammation (interleukin-6, IL-6), and oxidative stress (F2-isoprostanes, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and total antioxidant capacity (TAC)) were quantified.

RESULTS:

In the peripheral circulation, positive associations were observed between plasma folate and Cbl, and plasma TAC (P ≤ 0.01; P ≤ 0.01) and plasma NAD(H) (P ≤ 0.05; P ≤ 0.05) levels, respectively. Plasma folate was inversely associated with plasma Hcy concentrations (P ≤ 0.05); however, no statistically significant relationships were observed between plasma Hcy and plasma markers of inflammation, oxidative stress, or [NAD(H)]. Within the CNS plasma Hcy correlated positively with CSF IL-6 (P ≤ 0.01) and negatively with CSF NAD(H) (P ≤ 0.05) concentrations. An inverse association was observed between CSF folate and CSF levels of IL-6 (P ≤ 0.05). Unexpectedly, a positive association between CSF Cbl and CSF 8-OHdG levels was also found (P ≤ 0.01).

DISCUSSION:

These results indicate that folate and Cbl concentrations may influence the levels of oxidative damage, inflammation, and NAD(H), both systemically and within the CNS.

• Authors: Jade Guest, Ayse Bilgin, Bevan Hokin, Trevor A Mori, Kevin D Croft, Ross Grant
• Journal: Nutritional neuroscience (Nutr Neurosci) Vol. 18 Issue 8 Pg. 355-64 ( 2015) ISSN: 1476-8305 [Electronic] England
• PMID: 26263423 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Biomarkers
• F2-Isoprostanes
• Interleukin-6
• Homocysteine
• NAD
• 8-Hydroxy-2'-Deoxyguanosine
• Folic Acid
• Deoxyguanosine
• Vitamin B 12

Topics

• 8-Hydroxy-2'-Deoxyguanosine
• Adult
• Aged
• Antioxidants (metabolism)
• Biomarkers (blood, cerebrospinal fluid)
• Central Nervous System (drug effects, metabolism)
• Deoxyguanosine (analogs & derivatives, blood, cerebrospinal fluid)
• F2-Isoprostanes (blood, cerebrospinal fluid)
• Female
• Folic Acid (administration & dosage, blood, cerebrospinal fluid)
• Homocysteine (blood, cerebrospinal fluid)
• Humans
• Inflammation (blood)
• Interleukin-6 (blood, cerebrospinal fluid)
• Linear Models
• Male
• Middle Aged
• NAD (blood, cerebrospinal fluid)
• Oxidative Stress
• Vitamin B 12 (administration & dosage, blood, cerebrospinal fluid)