HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Binding of Multiple Rap1 Proteins Stimulates Chromosome Breakage Induction during DNA Replication.

Abstract
Telomeres, the ends of linear eukaryotic chromosomes, have a specialized chromatin structure that provides a stable chromosomal terminus. In budding yeast Rap1 protein binds to telomeric TG repeat and negatively regulates telomere length. Here we show that binding of multiple Rap1 proteins stimulates DNA double-stranded break (DSB) induction at both telomeric and non-telomeric regions. Consistent with the role of DSB induction, Rap1 stimulates nearby recombination events in a dosage-dependent manner. Rap1 recruits Rif1 and Rif2 to telomeres, but neither Rif1 nor Rif2 is required for DSB induction. Rap1-mediated DSB induction involves replication fork progression but inactivation of checkpoint kinase Mec1 does not affect DSB induction. Rap1 tethering shortens artificially elongated telomeres in parallel with telomerase inhibition, and this telomere shortening does not require homologous recombination. These results suggest that Rap1 contributes to telomere homeostasis by promoting chromosome breakage.
AuthorsGreicy H Goto, Sevil Zencir, Yukinori Hirano, Hiroo Ogi, Andreas Ivessa, Katsunori Sugimoto
JournalPLoS genetics (PLoS Genet) Vol. 11 Issue 8 Pg. e1005283 (Aug 2015) ISSN: 1553-7404 [Electronic] United States
PMID26263073 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • DNA, Fungal
  • RAP1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Shelterin Complex
  • Telomere-Binding Proteins
  • Transcription Factors
Topics
  • Chromosomes, Fungal (genetics)
  • DNA Breaks, Double-Stranded
  • DNA Replication
  • DNA, Fungal (genetics, metabolism)
  • Protein Binding
  • Saccharomyces cerevisiae (genetics, metabolism)
  • Saccharomyces cerevisiae Proteins (metabolism)
  • Shelterin Complex
  • Telomere Homeostasis
  • Telomere-Binding Proteins (metabolism)
  • Transcription Factors (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: