HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

GPX3 promoter is methylated in chronic myeloid leukemia.

Abstract
Hypermethylation of GPX3 (glutathione peroxidase 3) promoter has been identified in various cancers. However, the pattern of GPX3 promoter methylation in chronic myeloid leukemia (CML) remains unknown. Our study was aimed to investigate the methylation status of GPX3 promoter and its clinical relevance in CML. Real-time quantitative methylation-specific PCR and bisulfite sequencing PCR was performed to detect the level of GPX3 methylation in 80 CML patients and 44 controls. GPX3 promoter in CML patients was significantly methylated compared with controls (P = 0.007). GPX3 highly methylated patients showed significantly older age than GPX3 lowly methylated patients (P = 0.037). However, patients with GPX3 methylation had significantly lower white blood cells than those with low GPX3 methylation (P = 0.006). BCR-ABL transcript in GPX3 highly methylated patients was a little lower than that in GPX3 lowly methylated patients (P = 0.161). No significant differences were observed in the frequency of GPX3 methylation in the different stages of CML (P = 1.000). Significantly negative correlation was observed between GPX3 expression and GPX3 methylation (R = -0.442, P = 0.004). GPX3 mRNA level in K562 cell line was significantly increased after 5-aza-2'-deoxycytidine treatment, and GPX3 methylation level was decreased. GPX3 hypermethylation is frequent in CML and is negatively associated with its expression.
AuthorsDong-Ming Yao, Jing-Dong Zhou, Ying-Ying Zhang, Lei Yang, Xiang-Mei Wen, Jing Yang, Hong Guo, Qin Chen, Jiang Lin, Jun Qian
JournalInternational journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 8 Issue 6 Pg. 6450-7 ( 2015) ISSN: 1936-2625 [Electronic] United States
PMID26261521 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • BCR-ABL1 fusion protein, human
  • Enzyme Inhibitors
  • RNA, Messenger
  • Decitabine
  • GPX3 protein, human
  • Glutathione Peroxidase
  • DNA Modification Methylases
  • Fusion Proteins, bcr-abl
  • Azacitidine
Topics
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Azacitidine (analogs & derivatives, pharmacology)
  • Case-Control Studies
  • DNA Methylation (drug effects)
  • DNA Modification Methylases (antagonists & inhibitors, metabolism)
  • Decitabine
  • Enzyme Inhibitors (pharmacology)
  • Epigenesis, Genetic (drug effects)
  • Female
  • Fusion Proteins, bcr-abl (genetics)
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Neoplastic
  • Glutathione Peroxidase (genetics, metabolism)
  • Humans
  • K562 Cells
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive (enzymology, genetics)
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Promoter Regions, Genetic
  • RNA, Messenger (genetics)
  • Young Adult

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: