Abstract |
Oxidative stress and inflammation are the important pathological basis of atherogenesis. So, attenuating oxidative stress and inflammation has a very important significance in the prevention and treatment of atherosclerosis. The aim of present study was to investigate whether anti-atherosclerotic effect of Tongxinluo (TXL), a compound traditional Chinese medicine, is related to its anti-oxidation and anti- inflammation in human cardiac microvascular endothelial cells (HCMEC). We found that TXL treatment significantly reduced serum lipid levels and atherosclerotic plaque formation of apoE-deficient mice, and improved endothelial cell function as evidenced by increased expression of CD31 and eNOS. TXL pretreatment could abrogate the up-regulation of ROS and MDA induced by C16. Further experiments showed that the anti-oxidative effect of TXL may be related to inhibiting the expression of p22( phox), p47( phox) and HO-1 in HCMECs. We also found that TXL could inhibit the release of IL-1β and TNFα induced by C16, which is mediated by inhibiting the expression and activation of NF-κB. In conclusion, TXL decreases atherosclerotic plaque formation and improves endothelial cell function by inhibiting oxidative stress and inflammation in HCMECs. This finding provides a new molecular mechanism for the anti-atherosclerotic effect of TXL.
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Authors | Xiao-Li Wu, Bin Zheng, Li-Shuang Jin, Ruo-Nan Zhang, Ming He, Zhan Yang, Jin-Kun Wen |
Journal | International journal of clinical and experimental pathology
(Int J Clin Exp Pathol)
Vol. 8
Issue 6
Pg. 6323-33
( 2015)
ISSN: 1936-2625 [Electronic] United States |
PMID | 26261508
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoproteins E
- Drugs, Chinese Herbal
- tongxinluo
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Topics |
- Animals
- Apolipoproteins E
(deficiency, genetics)
- Atherosclerosis
(pathology)
- Blotting, Western
- Disease Models, Animal
- Drugs, Chinese Herbal
(pharmacology)
- Endothelial Cells
(drug effects)
- Enzyme-Linked Immunosorbent Assay
- Humans
- Immunohistochemistry
- Inflammation
(pathology)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Oxidative Stress
(drug effects)
- Reverse Transcriptase Polymerase Chain Reaction
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