Anti-allergic effect of α-cubebenoate isolated from Schisandra chinensis using in vivo and in vitro experiments.

Abstract

AIM OF THE STUDY:

In Oriental countries, the dried fruits of Schisandra chinensis are extensively used in traditional medicine to treat asthma, gonorrhea, and other diseases. Recently, α-cubebenoate was isolated as an anti-inflammatory component from Schisandra chinensis. In the present study, the authors examined the anti-allergic effect of α-cubebenoate using in vivo and in vitro experiments.

MATERIALS AND METHODS:

α-Cubebenoate was isolated from an extract of Schisandra chinensis fruits. Antigen-induced degranulation and Ca(2+) mobilization were measured in RBL-2H3 mast cells. In addition, BALB/c mice were sensitized with ovalbumin and aluminum hydroxide, and then challenged with ovalbumin for three consecutive days. α-Cubebenoate (1mg/kg) was administered intraperitoneally 30min before each ovalbumin challenge.

RESULTS:

In RBL-2H3 mast cells, α-cubebenoate inhibited antigen-induced degranulation and increase of intracellular Ca(2+) concentrations. In the ovalbumin-induced asthma model, α-cubebenoate suppressed bronchiolar structural changes induced by ovalbumin challenge. Furthermore, α-cubebenoate strongly inhibited accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar lavage fluid. α-Cubebenoate also suppressed Th2 cytokines (IL-4 and IL-13) and TGF-β1 in lung tissues and in immune cells at the mRNA and protein levels.

CONCLUSION:

α-Cubebenoate has an inhibitory effect on allergic inflammation and could be utilized as an agent for the treatment of asthma.

• Authors: Kyoung-Pil Lee, Saeromi Kang, Soo-Jin Park, Jung-Min Kim, Jung-Min Lee, Ae-Yeon Lee, Hae-Young Chung, Young-Whan Choi, Young-Geun Lee, Dong-Soon Im
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 173 Pg. 361-9 (Sep 15 2015) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 26253578 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Anti-Allergic Agents
• Anti-Inflammatory Agents
• Cytokines
• RNA, Messenger
• Sesquiterpenes, Guaiane
• alpha-cubebenoate
• Ovalbumin

Topics

• Animals
• Anti-Allergic Agents (pharmacology, therapeutic use)
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Asthma (drug therapy, immunology, pathology)
• Bronchoalveolar Lavage Fluid (cytology, immunology)
• Cell Count
• Cell Degranulation (drug effects)
• Cell Line
• Cytokines (genetics, immunology)
• Fruit
• Lung (drug effects, pathology)
• Male
• Mast Cells (drug effects, physiology)
• Mice, Inbred BALB C
• Ovalbumin
• RNA, Messenger (metabolism)
• Rats
• Schisandra
• Sesquiterpenes, Guaiane (pharmacology, therapeutic use)