Abstract | BACKGROUND: METHODS: Using two independent cohorts (cohort 1, n = 339 in total; cohort 2, n = 140 in total) consisting of human bladder tissues from BC patients and normal controls, we analyzed the gene expression levels of USP2a. A quantitative real-time PCR amplification was performed using a Rotor Gene 6000 instrument to quantify the expression of USP2a mRNA. RESULTS: A comparison of 305 bladder cancers and 34 age-matched controls showed an 81.4% reduction in USP2a expression in bladder cancers as compared to normal bladder tissues (p < 0.001). In the independent cohort consisting of 140 BC tissues and matched adjacent normal bladder tissues, the levels of USP2a in the specimens of BC patients were reduced by 86.9% as compared to matched surrounding normal specimens from the same patients (p < 0.001). Furthermore, there was 36.3% reduction of USP2a gene expression in muscle invasive bladder cancer (MIBC, n = 121), compared to non muscle invasive bladder cancer ( NMIBC, n = 184) (p = 0.004). Lastly, USP2a mRNA expression was significantly reduced in higher stages of MIBC patients (p = 0.024), but not in NMIBC patients. CONCLUSIONS: Our findings suggest that USP2a mRNA may be considered as a diagnostic marker candidate for bladder cancer, in particular, to stratify MIBC patients with a more invasive phenotype.
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Authors | Pildu Jeong, Yun-Sok Ha, Seok-Joong Yun, Hyung Yoon Yoon, Michael R Freeman, Jayoung Kim, Wun-Jae Kim |
Journal | BMC urology
(BMC Urol)
Vol. 15
Pg. 80
(Aug 07 2015)
ISSN: 1471-2490 [Electronic] England |
PMID | 26250800
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Endopeptidases
- USP2 protein, human
- Ubiquitin Thiolesterase
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Topics |
- Aged
- Biomarkers, Tumor
- Endopeptidases
(metabolism)
- Female
- Gene Expression Profiling
(methods)
- Humans
- Male
- Middle Aged
- Reproducibility of Results
- Sensitivity and Specificity
- Ubiquitin Thiolesterase
- Urinary Bladder Neoplasms
(diagnosis, metabolism)
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