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The quantitation of oxalate in amniotic fluid by ion-chromatography.

Abstract
Calcium oxalate is the predominant constituent of most kidney stones. The rare genetic disorder, primary hyperoxaluria, is characterized by the continuous excessive synthesis and urinary excretion of oxalic acid, leading to stone formation and renal insufficiency. The earliest measurement of oxalate in suspected cases of primary hyperoxaluria is advantageous and would lend support for continued analysis and eventual confirmation of the disease. Therefore, we quantitated oxalate levels in amniotic fluid (AF) using medium pressure ion-exchange chromatography. The mean concentration of oxalate in amniotic fluid was 1.67 mg/l +/- 0.8 (SD); (range 0.64 to 5.11 mg/l). The mean oxalate/creatinine ratio (O/C) was 0.23 +/- 0.11 (SD); (range 0.07 to 0.53). This ratio is similar to that found in the urine of infants less than 1 year (0.19 +/- 0.10; n = 17). There was no significant difference between males and females in oxalate concentration or O/C ratio. Regression analysis showed no significant correlation of fetal age with oxalate, O/C or creatinine. Studies in 13 sets of di-amnionic twins showed no statistical difference in oxalate or O/C between twin A and B. This study demonstrates the ability to accurately quantitate oxalate in amniotic fluid by ion-chromatography, and suggests that this may have a potential application in the initial screening process for the prenatal detection of primary hyperoxaluria.
AuthorsT R Wandzilak, F W Hanson, H E Williams
JournalClinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 185 Issue 2 Pg. 131-7 (Nov 1989) ISSN: 0009-8981 [Print] Netherlands
PMID2624994 (Publication Type: Journal Article)
Chemical References
  • Oxalates
Topics
  • Amniotic Fluid (analysis)
  • Female
  • Humans
  • Hyperoxaluria (diagnosis)
  • Hyperoxaluria, Primary (diagnosis)
  • Male
  • Oxalates (analysis)
  • Pregnancy
  • Prenatal Diagnosis (methods)

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