[The influences of ultrafiltration and alcohol sedimentation on protective effects of Radix Astragali and Radix Hedyseri against rat's cerebral ischemia].

Abstract

OBJECTIVE:

To investigate the influences of ultrafiltration and alcohol sedimentation on protective effects of Radix Astragali and Radix Hedyseri against rat's cerebral ischemia.

METHODS:

Using dexamethasone (im.) and ligating common carotid artery, the rat stasis model combined transient cerebral ischemia was established to evaluate the effects of the ultrafiltration and alcohol sedimentation through detecting antioxidant system and other indexes in brain tissue.

RESULTS:

The results showed that the 6 g/kg water extract(crude drug), ultrafiltration and alcohol sedimentation of Radix Astragali and Radix Hedyseri could upgrade adenosine-triphosphate (ATP), superoxide dismutase (SOD) and catalase (CAT), and degrade malondialdehyde(MDA) and water content of brain tissue in rat stasis model combined transient cerebral ischemia, the water extract and ultrafiltration of them could degrade lactic acid (LD) of brain tissue, and the effects of alcohol sedimentation of Radix Astragali and Radix Hedyseri become weaker than water extract of them.

CONCLUSION:

The water extract, ultrafiltration and alcohol sedimentation of Radix Astragali and Radix Hedyseri have some protective effects on cerebral ischemia in rats, the effective differences of the extract through the same extraction method are not remarkable, and alcohol precipitation method has obvious influences effect on Radix Astragali and Radix Hedyseri.

• Authors: Yong-qi Liu, Zhi-wang Wang, Shu-chang Wei, Chun-lu Yan, Rui-qiong Wang, Ying-dong Li
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 31 Issue 2 Pg. 132-5 (Mar 2015) ISSN: 1000-6834 [Print] China
• PMID: 26248418 (Publication Type: Journal Article)

Chemical References

• Alcohols
• Antioxidants
• Drugs, Chinese Herbal
• Malondialdehyde
• Huang Qi
• Catalase
• Superoxide Dismutase

Topics

• Alcohols (chemistry)
• Animals
• Antioxidants (metabolism)
• Astragalus Plant (chemistry)
• Astragalus propinquus
• Brain (drug effects)
• Catalase (metabolism)
• Cerebral Infarction (drug therapy)
• Drugs, Chinese Herbal (pharmacology)
• Malondialdehyde (metabolism)
• Plant Roots (chemistry)
• Rats
• Superoxide Dismutase (metabolism)
• Ultrafiltration