Characteristics of nonalcoholic fatty liver disease induced in wistar rats following four different diets.

Abstract

BACKGROUND/OBJECTIVES:

The prevalence of nonalcoholic fatty liver disease (NAFLD) has increased worldwide in parallel with overnutrition characterized by high-fat and high-carbohydrate intake. Our objective was to establish, in 16 weeks, a model of NAFLD in Wistar pathogen-free rats following four dietary types.

MATERIALS/METHODS:

Forty (6 weeks old) healthy Wistar male rats, weighing an average of 150 g were randomly divided into four groups of ten and assigned a diet with the same quantity (15 g/rat/day), but with different composition. The moderate-fat (MF) group was fed a moderate-fat diet (31.5% fat and 50% carbohydrates), the high-fat (HF) group was fed a fat-rich diet (51% fat), the high-sucrose (HS) group and the high-fructose (HFr) group were fed a carbohydrate-rich diet (61%). The carbohydrate contents of the HS group was composed of 60.3% sucrose while that of the HFr group was composed of 59.3% fructose.

RESULTS:

At week 16, the HF group had the highest percentage of cells enriched in fat (40%) and the highest weight and liver weight (P < 0.05). The HFr group showed significantly higher levels of serum triglycerides, alanine aminotransferase and adiponectin at week 16 as compared to week 1 (P < 0.05).

CONCLUSIONS:

The 15 g/rat/day diet composed of 51% fat or 61% carbohydrates enriched mainly in fructose may induce characteristics of NAFLD in rats.

• Authors: Nicole Fakhoury-Sayegh, Viviane Trak-Smayra, Aline Khazzaka, Fady Esseily, Omar Obeid, May Lahoud-Zouein, Hassan Younes
• Journal: Nutrition research and practice (Nutr Res Pract) Vol. 9 Issue 4 Pg. 350-7 (Aug 2015) ISSN: 1976-1457 [Print] Korea (South)
• PMID: 26244072 (Publication Type: Journal Article)