Inhibitory effect of group II mGluR agonist 2R, 4R-APDC on cell proliferation in dentate gyrus in rats with epileptic seizure.

Abstract

OBJECTIVE:

Epileptic seizure can increase the cell proliferation in dentate gyrus in brain, but the mechanism remains unclear.

MATERIALS AND METHODS:

In this study, using systemic bromodeoxyuridine (BrdU) to label the dividing cells, the inhibitory effect of group II metabotropic glutamate receptor (mGluR) agonist 2R, 4R-4-aminopyrrolidine-2, 4-dicarboxylate (2R, 4R-APDC) on cell proliferation in dentate gyrus in rats after pilocarpine-induced status epilepticus (SE) was investigated.

RESULTS:

Results found that, 2R, 4R-APDC could significantly inhibit the behavioral seizure and block the seizure-induced increase of BrdU-positive cells in dentate gyrus, especially in hilus. Double-label immunofluorescence staining showed that, 2R, 4R-APDC did not affect the ability of newborn cells to differentiate into neurons or astrocytes.

CONCLUSIONS:

2R, 4R-APDC not only has anticonvulsant effect on adult rats with pilocarpine-induced SE, but also has neuroprotective effect by reducing the abnormal regeneration of nerves.

• Authors: H Yao, Y-B Feng, Y-J Pang, J-J Xu, B-X Yu, X-P Liu
• Journal: European review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 19 Issue 15 Pg. 2922-7 (Aug 2015) ISSN: 2284-0729 [Electronic] Italy
• PMID: 26241549 (Publication Type: Journal Article)

Chemical References

• 4-aminopyrrolidine-2,4-dicarboxylic acid
• Anticonvulsants
• Excitatory Amino Acid Agonists
• Neuroprotective Agents
• Receptors, Metabotropic Glutamate
• metabotropic glutamate receptor 2
• Proline

Topics

• Animals
• Anticonvulsants (pharmacology, therapeutic use)
• Cell Proliferation (drug effects)
• Dentate Gyrus (drug effects, pathology)
• Epilepsy (drug therapy, pathology)
• Excitatory Amino Acid Agonists (pharmacology, therapeutic use)
• Male
• Neuroprotective Agents (pharmacology, therapeutic use)
• Proline (analogs & derivatives, pharmacology, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Receptors, Metabotropic Glutamate (agonists)