Synaptic connections of amacrine cells containing vesicular glutamate transporter 3 in baboon retinas.

Abstract

The goals of these experiments were to describe the morphology and synaptic connections of amacrine cells in the baboon retina that contain immunoreactive vesicular glutamate transporter 3 (vGluT3). These amacrine cells had the morphology characteristic of knotty bistratified type 1 cells, and their dendrites formed two plexuses on either side of the center of the inner plexiform layer. The primary dendrites received large synapses from amacrine cells, and the higher-order dendrites were both pre- and postsynaptic to other amacrine cells. Based on light microscopic immunolabeling results, these include AII cells and starburst cells, but not the polyaxonal amacrine cells tracer-coupled to ON parasol ganglion cells. The vGluT3 cells received input from ON bipolar cells at ribbon synapses and made synapses onto OFF bipolar cells, including the diffuse DB3a type. Many synapses from vGluT3 cells onto retinal ganglion cells were observed in both plexuses. At synapses where vGluT3 cells were presynaptic, two types of postsynaptic densities were observed; there were relatively thin ones characteristic of inhibitory synapses and relatively thick ones characteristic of excitatory synapses. In the light microscopic experiments with Neurobiotin-injected ganglion cells, vGluT3 cells made contacts with midget and parasol ganglion cells, including both ON and OFF types. Puncta containing immunoreactive gephyrin, an inhibitory synapse marker, were found at appositions between vGluT3 cells and each of the four types of labeled ganglion cells. The vGluT3 cells did not have detectable levels of immunoreactive γ-aminobutyric acid (GABA) or immunoreactive glycine transporter 1. Thus, the vGluT3 cells would be expected to have ON responses to light and make synapses onto neurons in both the ON and the OFF pathways. Taken with previous results, these findings suggest that vGluT3 cells release glycine at some of their output synapses and glutamate at others.

• Authors: David W Marshak, Alice Z Chuang, Drew M Dolino, Roy A Jacoby, Weiley S Liu, Y E Long, Michael B Sherman, Jae M Suh, Alejandro Vila, Stephen L Mills
• Journal: Visual neuroscience (Vis Neurosci) Vol. 32 Pg. E006 (Jan 2015) ISSN: 1469-8714 [Electronic] England
• PMID: 26241195 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Calbindins
• Vesicular Glutamate Transport Proteins
• neurobiotin
• gamma-Aminobutyric Acid
• Biotin
• Choline O-Acetyltransferase

Topics

• Amacrine Cells (metabolism, ultrastructure)
• Animals
• Biotin (analogs & derivatives, metabolism)
• Calbindins (metabolism)
• Choline O-Acetyltransferase (metabolism)
• Dendrites (ultrastructure)
• Microscopy, Confocal
• Microscopy, Immunoelectron
• Nerve Net (metabolism, ultrastructure)
• Papio (anatomy & histology)
• Retina (cytology)
• Synapses (physiology, ultrastructure)
• Vesicular Glutamate Transport Proteins (metabolism, ultrastructure)
• Visual Pathways (physiology)
• gamma-Aminobutyric Acid (metabolism)