Effects of a Tricaprylin Emulsion on Anti-glomerular Basement Membrane Glomerulonephritis in Rats: In Vivo and in Silico Studies.
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| Abstract |
Glomerulonephritis (GN) is a set of pathological conditions that result in the destruction of glomeruli and loss of renal function, commonly leading to the development of end-stage renal disease. Current pharmacotherapy is limited to immunosuppressive therapy. In the present study, we found a novel antinephritic effect of a tricaprylin emulsion in the anti-glomerular basement membrane (anti-GBM) GN rat model. We evaluated the treatment in vivo by comparing administration of the emulsion with administration of a casein kinase II (CK2) inhibitor in this rat model, and performed a gene ontology-based microarray analysis to reveal in silico the detailed mechanism of action. Our results showed that administration of the tricaprylin emulsion, or even tricaprylin alone, significantly ameliorated the anti-GBM antibody-induced renal dysfunction in these rats. We believe that tricaprylin is the key active antinephritic component of the emulsion and might be a promising drug for the effective treatment of nephritis. Moreover, with respect to microarray analysis, we developed a generally applicable and rapid method to compare gene expression profile data for multiple models of nephritis and clinical samples from a public domain microarray database.
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| Authors | Ning Liu, Junfeng Shi, Ying Xiao, Misato Yasue, Yoshinori Takei, Hayato Sanefuji, Gozoh Tsujimoto, Akira Hirasawa |
| Journal | Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 38 Issue 8 Pg. 1175-84 ( 2015) ISSN: 1347-5215 [Electronic] Japan |
| PMID | 26235580 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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