The objectives were to confirm that intravenous
fish oil (FO)
emulsions could alleviate
acute lung injury, modulate immunity, and reduce
inflammation in rats with abdominal
sepsis and to explore the mechanisms of these effects. Thirty-six adult male Sprague-Dawley rats were divided into 4 groups randomly. Two days after central venous catheterization, rats were subjected to cecal
ligation and
puncture to produce abdominal
sepsis. Rats were assigned to receive
normal saline or
total parenteral nutrition (TPN) containing standard
soybean oil emulsions or FO-supplemented TPN at the onset of
sepsis for 5 days. A
sham operation and control treatment were performed in control group rats.
Acute lung injury scores, peripheral blood lymphocyte subsets, plasma
cytokines, and Foxp3 expression in the spleen were determined. Compared with the
normal saline and TPN without FO, FO-supplemented TPN beneficially altered the distributions of the T-lymphocyte subsets and downregulated the
acute lung injury scores, plasma
cytokines, and expression of Foxp3 due to
sepsis.
Fish oil-supplemented TPN can decrease
acute lung injury scores, alleviate histopathology, reduce the bacterial load in the peritoneal lavage fluid, modulate the lymphocyte subpopulation in the peripheral blood, downregulate Foxp3 expression in the spleen, and reduce plasma
cytokines, which means that FO-supplemented TPN can alleviate
acute lung injury, modulate immunity, and reduce
inflammation in rats with abdominal
sepsis.