Pyruvate dose response studies targeting the vital signs following hemorrhagic shock.

Abstract

OBJECTIVES:

To determine the optimal effective dose of sodium pyruvate in maintaining the vital signs following hemorrhagic shock (HS) in rats.

MATERIALS AND METHODS:

Anesthetized, male Sprague-Dawley rats underwent computer-controlled HS for 30 minute followed by fluid resuscitation with either hypertonic saline, or sodium pyruvate solutions of 0.5 M, 1.0 M, 2.0 M, and 4.0 M at a rate of 5ml/kg/h (60 minute) and subsequent blood infusion (60 minute). The results were compared with sham and non- resuscitated groups. The animals were continuously monitored for mean arterial pressure, systolic and diastolic pressure, heart rate, pulse pressure, temperature, shock index and Kerdo index (KI).

RESULTS:

The Sham group remained stable throughout the experiment. Non-resuscitated HS animals did not survive for the entire experiment due to non-viable vital signs and poor shock and KI. All fluids were effective in normalizing the vital signs when shed blood was used adjunctively. Sodium pyruvate 2.0 M was most effective, and 4.0 M solution was least effective in improving the vital signs after HS.

CONCLUSIONS:

Future studies should be directed to use 2.0 M sodium pyruvate adjuvant for resuscitation on multiorgan failure and survival rate in HS.

• Authors: Pushpa Sharma, Makler Vyacheslav, Chalut Carissa, Rodriguez Vanessa, Mike Bodo
• Journal: Journal of emergencies, trauma, and shock (J Emerg Trauma Shock) 2015 Jul-Sep Vol. 8 Issue 3 Pg. 159-66 ISSN: 0974-2700 [Print] India
• PMID: 26229300 (Publication Type: Journal Article)