[Effect of Cynaropicrin on 2,3,4,7,8-Pentachlorodibenzofuran-induced Wasting Syndrome and Oxidative Stress].

Abstract

The effect of cynaropicrin that is the major component of an edible plant, artichoke (Cynara scolymus) on 2,3,4,7,8-pentachlorodibenzofuran (PenCDF)-induced toxicity in mice was studied. We evaluated the effect of cynaropicrin on the wasting syndrome and oxidative stress elicited by PenCDF. However, the PenCDF dose-response relationship on the wasting syndrome has been superficial. Therefore, we determined the dose which causes wasting syndrome in C57BL/6J mice, a responsive strain to dioxins. Since 2,3,7,8-tetrachlorodibenzo-p-dioxin (0.1 mg/kg, p.o.) induces hepatic ethoxyresorfin O-deethylase (EROD) activity in mice, we set the doses of PenCDF at 0.3, 1.0, 3.0, 5.0 and 10 mg/kg (once, p.o.) on the basis of its toxic-eqivalency factor (0.3). The wasting syndrome was evaluated by measuring the daily changes of body weight. Thiobarbituric acid-reactive substances were used as an index of oxidative stress. Of PenCDF doses examined, wasting syndrome and oxidative stress took place most markedly in 5 mg/kg. In disagreement with this, EROD activity which is the marker of the aryl hydrocarbon receptor-dependent induction of cytochrome P450 1a1 was elevated most abundantly at 0.3 mg/kg. Then, we examined the effect of cynaropicrin on the wasting syndrome and oxidative stress provoked by PenCDF at 5 mg/kg. However, this compound up to 20 mg/kg (p.o.) did not attenuate PenCDF-induced wasting syndrome. On the contray, PenCDF-induced oxidateive stress was suppressed by cynaropicrin at the highest dose (20 mg/kg), although EROD activity was increased rather than reduced by cynaropicrin at lower doses. Thus, it is suggested that cynaropicrin has an ability to reduce oxidative stress caused by PenCDF.

• Authors: Ken-ichi Yamada, Yuji Ishii, Tomoki Takeda, Hiroaki Kuroki, Chikage Mitoma, Hiroshi Uchi, Masutaka Furue, Hideyuki Yamada
• Journal: Fukuoka igaku zasshi = Hukuoka acta medica (Fukuoka Igaku Zasshi) Vol. 106 Issue 5 Pg. 169-75 (May 2015) ISSN: 0016-254X [Print] Japan
• PMID: 26226680 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzofurans
• Lactones
• Sesquiterpenes
• cynaropicrin
• 2,3,4,7,8-pentachlorodibenzofuran

Topics

• Animals
• Benzofurans (administration & dosage)
• Dose-Response Relationship, Drug
• Lactones (pharmacology)
• Mice
• Mice, Inbred C57BL
• Oxidative Stress (drug effects)
• Sesquiterpenes (pharmacology)
• Wasting Syndrome (chemically induced)