Effect of Pin1 inhibitor juglone on proliferation, migration and angiogenic ability of breast cancer cell line MCF7Adr.

Abstract

This study aimed to evaluate the effects of Pin1 inhibitor Juglone on proliferation, migration and the angiogenic ability of breast cancer cell line MCF7Adr. MCF7Adr cells were cultured and separately treated with Pin1 inhibitor Juglone (treatment group) and DMEM without drug (control group). The cell cycle was examined by flow cytometry. Cell migration was measured by wound-healing assay. Cyclin E protein content was detected by Western blotting. The angiogenesis factor vascular endothelial growth factor (VEGF) in cell media was determined by enzyme linked immunosorbent assay. The results showed that the percentage of cells in G2/M phase in treatment group was significantly higher than that in control group (25.5% vs. 10.1%, P<0.05), and that in G0/G1 phase and S stage in treatment group was significantly lower than that in control group (40.5% vs. 48.2%, and 33.7% vs. 41.7%, P<0.05). Cyclin E protein content in treatment group was significantly lower than that in control group (39.2 ± 7.4 vs. 100 ± 23.1, P<0.05). (A0-A24)/A0 value in treatment group was significantly lower than that in control group (23.9 ± 3.8 vs. 100 ± 14.4, P<0.05). VEGF-A, -B, and -C contents in cell media of treatment group were significantly lower than those in control group (P<0.05). It was suggested that Pin1 inhibitor Juglone can effectively inhibit the proliferation, migration and the angiogenic ability of MCF7Adr cells, and can be used as an alternative drug therapy for breast cancer.

• Authors: Yuan-Gui Hu, Yun-Feng Shen, Yi Li
• Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 35 Issue 4 Pg. 531-534 (Aug 2015) ISSN: 1993-1352 [Electronic] China
• PMID: 26223922 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Cyclin E
• NIMA-Interacting Peptidylprolyl Isomerase
• Naphthoquinones
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• PIN1 protein, human
• Peptidylprolyl Isomerase
• juglone

Topics

• Antineoplastic Agents (pharmacology)
• Breast Neoplasms (drug therapy, metabolism)
• Cell Cycle (drug effects)
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Cyclin E (metabolism)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• MCF-7 Cells
• NIMA-Interacting Peptidylprolyl Isomerase
• Naphthoquinones (pharmacology)
• Peptidylprolyl Isomerase (antagonists & inhibitors)
• Vascular Endothelial Growth Factor A (metabolism)