Multifunctional fluorescent magnetic nanoparticles for lung cancer stem cells research.

Abstract

In this paper, a multifunctional peptide-fluorescent-magnetic nanocomposites (Fe₃O₄@PEI@Cy5.5@PEG@HCBP-1 NPs) was synthesized via a layer-by-layer approach for potential application to cancer diagnoses. The multifunctional nanocomposites have great dispersibility and homogeneous particle sizes in aqueous solution. Meanwhile, it has perfect hemocompatibility and satisfying cytocompatibility in a relatively high concentration. Data from in vitro cytotoxicity assay indicated that the nanocomposites could recognize the lung cancer stem cells (CSCs) specifically and enrich the HCBP-1 positive CSCs from H460 tumor xenografts effectively. Additionally, the results of in vivo live fluorescent imaging and magnetic resonance imaging (MRI) showed that the nanocomposites could identify lung CSCs in tumor xenografts. These results suggested that the nanocomposites could be used as a potential cancer diagnostic agent through modifying diverse fluorescence dyes and targeting ligands on its surface.

• Authors: Xuan Zhou, Lisha Chen, Anxin Wang, Yufei Ma, Hailu Zhang, Yimin Zhu
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 134 Pg. 431-9 (Oct 01 2015) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 26222409 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Topics

• Cell Line, Tumor
• Fluorescence
• Hemolysis
• Humans
• Lung Neoplasms (pathology)
• Magnetics
• Microscopy, Electron, Transmission
• Nanoparticles
• Neoplastic Stem Cells (pathology)
• X-Ray Diffraction