Noninvasive theranostic imaging of HSV-TK/GCV suicide gene therapy in liver cancer by folate-targeted quantum dot-based liposomes.

Abstract

Theranostics is emerging as a popular strategy for cancer therapy; thanks to the development of nanotechnology. In this work, we have combined an HSV-TK/GCV suicide gene system and near-infrared quantum dots, as the former is quite effective in liver cancer treatment and the latter facilitates tumor imaging. A folate-modified theranostic liposome (FL/QD-TK) was developed, which is composed of an HSV-TK suicide gene covalently coupling with near-infrared fluorescent CdSeTe/ZnS core/shell quantum dots. The liver cancer-targeting and biosafety of FL/QD-TK were studied in vitro and in vivo. FL/QD-TK exhibited highly specific tumor imaging and strong inhibition of the folate receptor-overexpressed Bel-7402 mouse xenografts without systematic toxicity. This study may shed light on gene delivery and targeted cancer therapy.

• Authors: Dan Shao, Jing Li, Yue Pan, Xin Zhang, Xiao Zheng, Zheng Wang, Ming Zhang, Hong Zhang, Li Chen
• Journal: Biomaterials science (Biomater Sci) Vol. 3 Issue 6 Pg. 833-41 (Jun 2015) ISSN: 2047-4849 [Electronic] England
• PMID: 26221843 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• Liposomes
• Folic Acid
• Thymidine Kinase
• Ganciclovir

Topics

• Animals
• Antiviral Agents (chemistry, pharmacology)
• Bile Ducts, Intrahepatic (chemistry, drug effects)
• Diagnostic Imaging
• Folic Acid (chemistry)
• Ganciclovir (chemistry, pharmacology)
• Gene Transfer Techniques
• Genes, Transgenic, Suicide (drug effects, genetics)
• Genetic Therapy (methods)
• Hep G2 Cells (chemistry, drug effects)
• Humans
• Liposomes
• Liver Neoplasms (chemistry, drug therapy)
• Mice
• Nanotechnology
• Quantum Dots (chemistry)
• Simplexvirus (drug effects, genetics)
• Theranostic Nanomedicine
• Thymidine Kinase (chemistry, genetics)