Bacterial membranes enhance the immunogenicity and protective capacity of the surface exposed tick Subolesin-Anaplasma marginale MSP1a chimeric antigen.

Abstract

Ticks are vectors of diseases that affect humans and animals worldwide. Tick vaccines have been proposed as a cost-effective and environmentally sound alternative for tick control. Recently, the Rhipicephalus microplus Subolesin (SUB)-Anaplasma marginale MSP1a chimeric antigen was produced in Escherichia coli as membrane-bound and exposed protein and used to protect vaccinated cattle against tick infestations. In this research, lipidomics and proteomics characterization of the E. coli membrane-bound SUB-MSP1a antigen showed the presence of components with potential adjuvant effect. Furthermore, vaccination with membrane-free SUB-MSP1a and bacterial membranes containing SUB-MSP1a showed that bacterial membranes enhance the immunogenicity of the SUB-MSP1a antigen in animal models. R. microplus female ticks were capillary-fed with sera from pigs orally immunized with membrane-free SUB, membrane bound SUB-MSP1a and saline control. Ticks ingested antibodies added to the blood meal and the effect of these antibodies on reduction of tick weight was shown for membrane bound SUB-MSP1a but not SUB when compared to control. Using the simple and cost-effective process developed for the purification of membrane-bound SUB-MSP1a, endotoxin levels were within limits accepted for recombinant vaccines. These results provide further support for the development of tick vaccines using E. coli membranes exposing chimeric antigens such as SUB-MSP1a.

• Authors: Marinela Contreras, Juan A Moreno-Cid, Ana Domingos, Mario Canales, Iratxe Díez-Delgado, José M Pérez de la Lastra, Emilio Sánchez, Octávio Merino, Rigoberto López Zavala, Nieves Ayllón, Mariana Boadella, Margarita Villar, Christian Gortázar, José de la Fuente
• Journal: Ticks and tick-borne diseases (Ticks Tick Borne Dis) Vol. 6 Issue 6 Pg. 820-8 (Sep 2015) ISSN: 1877-9603 [Electronic] Netherlands
• PMID: 26219233 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier GmbH. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Antibodies
• Antigens
• Arthropod Proteins
• Bacterial Outer Membrane Proteins
• Vaccines
• major surface protein 1a, Anaplasma
• subolesin, tick

Topics

• Adjuvants, Immunologic
• Anaplasma marginale (immunology)
• Animals
• Antibodies (blood)
• Antigens (immunology)
• Arthropod Proteins (immunology)
• Bacterial Outer Membrane Proteins (immunology)
• Cell Membrane (chemistry)
• Escherichia coli
• Female
• Mice
• Mice, Inbred BALB C
• Rabbits
• Rhipicephalus (immunology)
• Swine
• Vaccines (immunology)