Abstract |
Caveolins are specific proteins involved in regulation of signal transduction to intracellular space. Still, their contribution to immune functions has not been completely clarified. Thus, we decided to characterize the expression of caveolins in bone marrow-derived macrophages (BMDMs) under resting and inflammatory conditions. The effect of classical activators ( lipopolysaccharide, LPS; interferon-gamma, IFN-γ) was further potentiated with hypoxic (5% O2) conditions. The activation of p44/42- extracellular signal-regulated kinases 1 and 2 (ERK1/2) and expression of caveolin-1, -2, and -3, hypoxia inducible factor-1 alpha (HIF-1α), as well as inducible nitric oxide synthase (iNOS) was monitored using the Western blot technique. The production of nitric oxide (NO) and tumor necrosis factor-alpha (TNFα) was analyzed by Griess method or ELISA, respectively. BMDMs were also transfected with siRNA against caveolin-2. Importantly, our study showed for the first time that BMDMs expressed only caveolin-2, and its level decreased after activation of macrophages with LPS, IFN-γ, and/or hypoxia. The expression of caveolin-2 negatively correlates with the iNOS and HIF-1α protein levels, as well as with the LPS/IFN-γ- and hypoxia-induced activation of ERK1/2. We concluded that caveolin-2 is most probably involved in regulation of pro-inflammatory responses of BMDMs, triggered via activation of ERK1/2.
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Authors | Michaela Maceckova, Hana Martiskova, Adolf Koudelka, Lukas Kubala, Antonin Lojek, Michaela Pekarova |
Journal | Immunobiology
(Immunobiology)
Vol. 220
Issue 11
Pg. 1266-74
(Nov 2015)
ISSN: 1878-3279 [Electronic] Netherlands |
PMID | 26215374
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier GmbH. All rights reserved. |
Chemical References |
- Caveolin 2
- Cytokines
- RNA, Small Interfering
- Nitric Oxide
- Nitric Oxide Synthase Type II
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 3
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Topics |
- Animals
- Caveolin 2
(genetics, metabolism)
- Cell Survival
- Cytokines
(metabolism)
- Gene Expression
- Gene Expression Regulation
- Hypoxia
(metabolism)
- Inflammation
(genetics, immunology, metabolism)
- Macrophage Activation
(genetics, immunology)
- Macrophages
(immunology, metabolism)
- Male
- Mice
- Mitogen-Activated Protein Kinase 1
(metabolism)
- Mitogen-Activated Protein Kinase 3
(metabolism)
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase Type II
(genetics, metabolism)
- Phenotype
- RNA Interference
- RNA, Small Interfering
(genetics)
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